Nature of bacterial colonization influences transcription of mucin genes in mice during the first week of life
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Anders Bergström, Matilde Bylov Kristensen, Martin Iain Bahl, Stine Broeng Metzdorff, Lisbeth Nielsen Fink, Hanne Frøkiær, Tine Rask Licht
Postnatal regulation of the small intestinal mucus layer is potentially important in the development of adult gut functionality. We hypothesized that the nature of bacterial colonization affects mucus gene regulation in early life.We thus analyzed the influence of the presence of a conventional microbiota as well as two selected monocolonizing bacterial strains on the transcription of murine genes involved in mucus layer development during the first week of life.Mouse pups (N = 8/group) from differently colonized dams: Germ-free (GF), conventional specific pathogen free (SPF), monocolonized with either Lactobacillus acidophilus NCFM (Lb) or Escherichia coli Nissle (Ec) were analyzed by qPCR on isolated ileal tissue sections from postnatal days 1 and 6 (PND1, PND6) after birth with respect to: (i) transcription of specific genes involved in mucus production (Muc1-4, Tff3) and (ii) amounts of 16S rRNA of Lactobacillus and E. coli. Quantification of 16S rRNA genes was performed to obtain a measure for amounts of colonized bacteria.
|Tidsskrift||BMC Research Notes|
|Status||Udgivet - 2012|
- Germ free mice, Monocolonized, qPCR, LinRegPCR, Postnatal transcription onset, Probiotics, Lactobacillus acidophilus NCFM, Escherichia coli Nissle, 16S rRNA