Purification, characterization and ELISA detection of mink immunoglobulins

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Cyril Jean-Marie Martel, Bent Aasted

This study describes easy purification methods for mink IgG, IgA and IgM immunoglobulins. IgG and IgM were purified from normal mink serum, while IgA was purified from mink bile from healthy animals. By SDS-polyacrylamid-gel-electrophoresis  (SDS-PAGE) and immunoblotting under reducing conditions the estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa respectively. The purities of purified IgG, IgM and IgA were estimated by immunoglobulin class specific ELISAs to be more than 90% for IgG and IgM, and more than 80% for IgA.
OriginalsprogEngelsk
TidsskriftScientifur
Vol/bind32
Udgave nummer1
Sider (fra-til)33-40
Antal sider8
ISSN0105-2403
StatusUdgivet - 2008

ID: 8297148