Widespread evolutionary conservation of alternatively spliced exons in caenorhabditis.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Manuel Irimia, Jakob L Rukov, David Penny, Jordi Garcia-Fernandez, Jeppe Vinther, Scott W Roy

Alternative splicing (AS) contributes to increased transcriptome and proteome diversity in various eukaryotic lineages. Previous studies showed low levels of conservation of alternatively spliced (cassette) exons within mammals and within dipterans. We report a strikingly different pattern in Caenorhabditis nematodes-more than 92% of cassette exons from Caenorhabditis elegans are conserved in Caenorhabditis briggsae and/or Caenorhabditis remanei. High levels of conservation extend to minor-form exons (present in a minority of transcripts) and are particularly pronounced for exons showing complex patterns of splicing. The functionality of the vast majority of cassette exons is underscored by various other features. We suggest that differences in conservation between lineages reflect differences in levels of functionality and further suggest that these differences are due to differences in intron length and the strength of consensus boundaries across lineages. Finally, we demonstrate an inverse relationship between AS and gene duplication, suggesting that the latter may be primarily responsible for the emergence of new functional transcripts in nematodes.
Udgivelsesdato: 2008-Feb
TidsskriftMolecular Biology and Evolution
Udgave nummer2
Sider (fra-til)375-82
Antal sider7
StatusUdgivet - 2007

ID: 2704231