Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Yilmaz Emre Gencay, Tina Birk, Martine Camilla Holst Sørensen, Lone Brøndsted

Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.

OriginalsprogEngelsk
TitelCampylobacter jejuni : Methods and Protocols
Antal sider10
Vol/bind1512
ForlagHumana Press
Publikationsdato2017
Sider19-28
ISBN (Trykt)978-1-4939-6534-2
ISBN (Elektronisk)978-1-4939-6536-6
DOI
StatusUdgivet - 2017
NavnMethods in Molecular Biology
Vol/bind1512
ISSN1064-3745

ID: 184356560