Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism

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Standard

Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism. / Weiss, Gudrun Margarethe; Rasmussen, Simon; Zeuthen, Louise; Nielsen, Birgit Margrethe Nøhr; Jarmer, Hanne Østergaard; Jespersen, Lene; Frøkiær, Hanne.

I: Immunology, Bind 131, Nr. 2, 2010, s. 268-281.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Weiss, GM, Rasmussen, S, Zeuthen, L, Nielsen, BMN, Jarmer, HØ, Jespersen, L & Frøkiær, H 2010, 'Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism', Immunology, bind 131, nr. 2, s. 268-281. https://doi.org/10.1111/j.1365-2567.2010.03301.x

APA

Weiss, G. M., Rasmussen, S., Zeuthen, L., Nielsen, B. M. N., Jarmer, H. Ø., Jespersen, L., & Frøkiær, H. (2010). Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism. Immunology, 131(2), 268-281. https://doi.org/10.1111/j.1365-2567.2010.03301.x

Vancouver

Weiss GM, Rasmussen S, Zeuthen L, Nielsen BMN, Jarmer HØ, Jespersen L o.a. Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism. Immunology. 2010;131(2):268-281. https://doi.org/10.1111/j.1365-2567.2010.03301.x

Author

Weiss, Gudrun Margarethe ; Rasmussen, Simon ; Zeuthen, Louise ; Nielsen, Birgit Margrethe Nøhr ; Jarmer, Hanne Østergaard ; Jespersen, Lene ; Frøkiær, Hanne. / Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism. I: Immunology. 2010 ; Bind 131, Nr. 2. s. 268-281.

Bibtex

@article{49fc02f089b611df928f000ea68e967b,
title = "Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism",
abstract = "Lactobacilli are probiotics that, among other health-promoting effects, have been ascribed immunostimulating and virus-preventive properties. Certain Lactobacillus spp. have been shown to possess strong interleukin-12 (IL-12) -inducing properties. As IL-12 production depends on the upregulation of type I interferons (IFNs), we hypothesized that the strong IL-12-inducing capacity of Lactobacillus acidophilus NCFM in murine bone-marrow-derived dendritic cells (DCs) is caused by an up-regulation of IFN-{\ss}, which subsequently induces IL-12 and the double-stranded RNA binding Toll-like receptor-3 (TLR-3). The expression of the genes encoding IFN-{\ss}, TLR-3, IL-12 and IL-10 in DCs upon stimulation with L. acidophilus NCFM was determined. Lactobacillus acidophilus NCFM induced a much stronger expression of Ifn-{\ss}, Il-12 and Il-10 compared with the synthetic double-stranded RNA ligand Poly I:C, whereas the levels of expressed Tlr-3 were similar. Whole genome microarray gene expression analysis revealed that other genes related to viral defence were significantly up-regulated and among the strongest induced genes in DCs stimulated with L. acidophilus NCFM. The ability to induce IFN-b was also detected in another L. acidophilus strain (X37), but was not a property of other probiotic strains tested, i.e. Bifidobacterium bifidum Z9 and Escherichia coli Nissle 1917. The IFN-{\ss} expression was markedly reduced in TLR-2)-/- DCs, dependent on endocytosis, and the major cause of the induction of Il-12 and Tlr-3 in DCs stimulated with L. acidophilus NCFM. Collectively, our results reveal that certain lactobacilli trigger the expression of viral defence genes in DCs in a TLR-2 manner dependent on IFN-{\ss}.",
author = "Weiss, {Gudrun Margarethe} and Simon Rasmussen and Louise Zeuthen and Nielsen, {Birgit Margrethe N{\o}hr} and Jarmer, {Hanne {\O}stergaard} and Lene Jespersen and Hanne Fr{\o}ki{\ae}r",
year = "2010",
doi = "10.1111/j.1365-2567.2010.03301.x",
language = "English",
volume = "131",
pages = "268--281",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism

AU - Weiss, Gudrun Margarethe

AU - Rasmussen, Simon

AU - Zeuthen, Louise

AU - Nielsen, Birgit Margrethe Nøhr

AU - Jarmer, Hanne Østergaard

AU - Jespersen, Lene

AU - Frøkiær, Hanne

PY - 2010

Y1 - 2010

N2 - Lactobacilli are probiotics that, among other health-promoting effects, have been ascribed immunostimulating and virus-preventive properties. Certain Lactobacillus spp. have been shown to possess strong interleukin-12 (IL-12) -inducing properties. As IL-12 production depends on the upregulation of type I interferons (IFNs), we hypothesized that the strong IL-12-inducing capacity of Lactobacillus acidophilus NCFM in murine bone-marrow-derived dendritic cells (DCs) is caused by an up-regulation of IFN-ß, which subsequently induces IL-12 and the double-stranded RNA binding Toll-like receptor-3 (TLR-3). The expression of the genes encoding IFN-ß, TLR-3, IL-12 and IL-10 in DCs upon stimulation with L. acidophilus NCFM was determined. Lactobacillus acidophilus NCFM induced a much stronger expression of Ifn-ß, Il-12 and Il-10 compared with the synthetic double-stranded RNA ligand Poly I:C, whereas the levels of expressed Tlr-3 were similar. Whole genome microarray gene expression analysis revealed that other genes related to viral defence were significantly up-regulated and among the strongest induced genes in DCs stimulated with L. acidophilus NCFM. The ability to induce IFN-b was also detected in another L. acidophilus strain (X37), but was not a property of other probiotic strains tested, i.e. Bifidobacterium bifidum Z9 and Escherichia coli Nissle 1917. The IFN-ß expression was markedly reduced in TLR-2)-/- DCs, dependent on endocytosis, and the major cause of the induction of Il-12 and Tlr-3 in DCs stimulated with L. acidophilus NCFM. Collectively, our results reveal that certain lactobacilli trigger the expression of viral defence genes in DCs in a TLR-2 manner dependent on IFN-ß.

AB - Lactobacilli are probiotics that, among other health-promoting effects, have been ascribed immunostimulating and virus-preventive properties. Certain Lactobacillus spp. have been shown to possess strong interleukin-12 (IL-12) -inducing properties. As IL-12 production depends on the upregulation of type I interferons (IFNs), we hypothesized that the strong IL-12-inducing capacity of Lactobacillus acidophilus NCFM in murine bone-marrow-derived dendritic cells (DCs) is caused by an up-regulation of IFN-ß, which subsequently induces IL-12 and the double-stranded RNA binding Toll-like receptor-3 (TLR-3). The expression of the genes encoding IFN-ß, TLR-3, IL-12 and IL-10 in DCs upon stimulation with L. acidophilus NCFM was determined. Lactobacillus acidophilus NCFM induced a much stronger expression of Ifn-ß, Il-12 and Il-10 compared with the synthetic double-stranded RNA ligand Poly I:C, whereas the levels of expressed Tlr-3 were similar. Whole genome microarray gene expression analysis revealed that other genes related to viral defence were significantly up-regulated and among the strongest induced genes in DCs stimulated with L. acidophilus NCFM. The ability to induce IFN-b was also detected in another L. acidophilus strain (X37), but was not a property of other probiotic strains tested, i.e. Bifidobacterium bifidum Z9 and Escherichia coli Nissle 1917. The IFN-ß expression was markedly reduced in TLR-2)-/- DCs, dependent on endocytosis, and the major cause of the induction of Il-12 and Tlr-3 in DCs stimulated with L. acidophilus NCFM. Collectively, our results reveal that certain lactobacilli trigger the expression of viral defence genes in DCs in a TLR-2 manner dependent on IFN-ß.

U2 - 10.1111/j.1365-2567.2010.03301.x

DO - 10.1111/j.1365-2567.2010.03301.x

M3 - Journal article

VL - 131

SP - 268

EP - 281

JO - Immunology

JF - Immunology

SN - 0019-2805

IS - 2

ER -

ID: 20686226