DNA from keratinous tissue. Part II: feather

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Maia E. Olsen, Camilla Friis Bengtsson, Mads Frost Bertelsen, Eske Willerslev, Tom Gilbert

Although good quality DNA can be recovered from the base of the calamus of freshly sampled feathers, as from other fully keratinized tissues such as nail or hair shaft, the quality and quantity of DNA in the majority of feather structures is much poorer. Little research has been performed to characterize the quality of this DNA is, and thus what a researcher might be able to achieve when using feathers as a source of DNA. In this review, we expand on our companion article detailing the quality of DNA in nail and hair, by synthesizing published, and new preliminary genetic data obtained from feathers. As with nail and hair, we demonstrate that although DNA can, in general, be recovered from all parts of the feather, the quality of such DNA varies. As such, although one can expect a priori that genetic analyses are possible on the feather, for PCR based analyses, it is extremely difficult to predict the size of amplicon that can be used in such analyses. However, PCR-free genetic analyses that can exploit much smaller DNA fragments may promise to be a powerful tool for future exploitation.
OriginalsprogEngelsk
TidsskriftAnnals of Anatomy
Vol/bind194
Udgave nummer1
Sider (fra-til)31-35
Antal sider5
ISSN0940-9602
DOI
StatusUdgivet - 2012

ID: 34347810