For a large variety of studies, it would be advantageous to be able to synthesize a library of individual peptide nucleic acid (PNA) oligomers in parallel. This is especially useful for screening target positions for antisense reagents along the mRNA (gene walk) as optimal target sites have to be determined emperically to a very large degree. Furthermore, because of the very limited cellular uptake of PNA oligomers in both eukaryotic and bacterial cells and the finding that conjugation of certain relatively simple peptides to the PNA may greatly improve the uptake, it is also of interest to be able to synthesize libraries of PNA-peptide conjugate (see Chapter 3). Methods have previously been described for the synthesis of PNA parallel libraries on membranes using Fmoc protection chemistry (1,2). This chapter describes a protocol for semi-automated parallel synthesis of up to 96-PNA-peptide conjugates (Table 1) at 0.5 μMole-scale (3).