TY - JOUR

T1 - Pancreatic beta-cells respond to fuel pressure with an early metabolic switch

AU - Malinowski, Ronja M.

AU - Ghiasi, Seyed M.

AU - Mandrup-Poulsen, Thomas

AU - Meier, Sebastian

AU - Lerche, Mathilde H.

AU - Ardenkjaer-Larsen, Jan H.

AU - Jensen, Pernille R.

PY - 2020

Y1 - 2020

N2 - Pancreatic beta-cells become irreversibly damaged by long-term exposure to excessive glucose concentrations and lose their ability to carry out glucose stimulated insulin secretion (GSIS) upon damage. The beta-cells are not able to control glucose uptake and they are therefore left vulnerable for endogenous toxicity from metabolites produced in excess amounts upon increased glucose availability. In order to handle excess fuel, the beta-cells possess specific metabolic pathways, but little is known about these pathways. We present a study of beta-cell metabolism under increased fuel pressure using a stable isotope resolved NMR approach to investigate early metabolic events leading up to beta-cell dysfunction. The approach is based on a recently described combination of C-13 metabolomics combined with signal enhanced NMR via dissolution dynamic nuclear polarization (dDNP). Glucose-responsive INS-1 beta-cells were incubated with increasing concentrations of [U-C-13] glucose under conditions where GSIS was not affected (2-8 h). We find that pyruvate and DHAP were the metabolites that responded most strongly to increasing fuel pressure. The two major divergence pathways for fuel excess, the glycerolipid/fatty acid metabolism and the polyol pathway, were found not only to operate at unchanged rate but also with similar quantity.

AB - Pancreatic beta-cells become irreversibly damaged by long-term exposure to excessive glucose concentrations and lose their ability to carry out glucose stimulated insulin secretion (GSIS) upon damage. The beta-cells are not able to control glucose uptake and they are therefore left vulnerable for endogenous toxicity from metabolites produced in excess amounts upon increased glucose availability. In order to handle excess fuel, the beta-cells possess specific metabolic pathways, but little is known about these pathways. We present a study of beta-cell metabolism under increased fuel pressure using a stable isotope resolved NMR approach to investigate early metabolic events leading up to beta-cell dysfunction. The approach is based on a recently described combination of C-13 metabolomics combined with signal enhanced NMR via dissolution dynamic nuclear polarization (dDNP). Glucose-responsive INS-1 beta-cells were incubated with increasing concentrations of [U-C-13] glucose under conditions where GSIS was not affected (2-8 h). We find that pyruvate and DHAP were the metabolites that responded most strongly to increasing fuel pressure. The two major divergence pathways for fuel excess, the glycerolipid/fatty acid metabolism and the polyol pathway, were found not only to operate at unchanged rate but also with similar quantity.

KW - ISOTOPE-RESOLVED METABOLOMICS

KW - STIMULATED INSULIN-SECRETION

KW - GLUCOSE

KW - LINE

KW - PATHWAY

KW - EXCESS

U2 - 10.1038/s41598-020-72348-1

DO - 10.1038/s41598-020-72348-1

M3 - Journal article

C2 - 32963286

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 15413

ER -