Pancreatic beta-cells respond to fuel pressure with an early metabolic switch

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Dokumenter

  • Ronja M. Malinowski
  • Seyed M. Ghiasi
  • Mandrup-Poulsen, Thomas
  • Sebastian Meier
  • Mathilde H. Lerche
  • Jan H. Ardenkjaer-Larsen
  • Pernille R. Jensen

Pancreatic beta-cells become irreversibly damaged by long-term exposure to excessive glucose concentrations and lose their ability to carry out glucose stimulated insulin secretion (GSIS) upon damage. The beta-cells are not able to control glucose uptake and they are therefore left vulnerable for endogenous toxicity from metabolites produced in excess amounts upon increased glucose availability. In order to handle excess fuel, the beta-cells possess specific metabolic pathways, but little is known about these pathways. We present a study of beta-cell metabolism under increased fuel pressure using a stable isotope resolved NMR approach to investigate early metabolic events leading up to beta-cell dysfunction. The approach is based on a recently described combination of C-13 metabolomics combined with signal enhanced NMR via dissolution dynamic nuclear polarization (dDNP). Glucose-responsive INS-1 beta-cells were incubated with increasing concentrations of [U-C-13] glucose under conditions where GSIS was not affected (2-8 h). We find that pyruvate and DHAP were the metabolites that responded most strongly to increasing fuel pressure. The two major divergence pathways for fuel excess, the glycerolipid/fatty acid metabolism and the polyol pathway, were found not only to operate at unchanged rate but also with similar quantity.

OriginalsprogEngelsk
Artikelnummer15413
TidsskriftScientific Reports
Vol/bind10
Udgave nummer1
Antal sider11
ISSN2045-2322
DOI
StatusUdgivet - 2020

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