Amphipols from A to Z

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • J-L Popot
  • T Althoff
  • D Bagnard
  • J-L Banères
  • P Bazzacco
  • E Billon-Denis
  • Laurent J. Catoire
  • P Champeil
  • D Charvolin
  • M J Cocco
  • G Crémel
  • T Dahmane
  • L M de la Maza
  • C Ebel
  • F Gabel
  • F Giusti
  • Y Gohon
  • E Goormaghtigh
  • E Guittet
  • J. H. Kleinschmidt
  • W Kühlbrandt
  • C Le Bon
  • M Picard
  • B Pucci
  • J N Sachs
  • C Tribet
  • C Van Heijenoort
  • F Wien
  • F Zito
  • M Zoonens
Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.
TidsskriftAnnual Review of Biophysics
Sider (fra-til)379-408
Antal sider30
StatusUdgivet - jun. 2011

ID: 33826425