Urea transport in human red blood cells: Donor variation compared to chloride, glucose, and water transport
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Urea transport in human red blood cells : Donor variation compared to chloride, glucose, and water transport. / Leifelt, Jonas; Dziegiel, Morten Hanefeld; Brahm, Jesper.
In: The Journal of general physiology, Vol. 155, No. 10, e202213321, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Urea transport in human red blood cells
T2 - Donor variation compared to chloride, glucose, and water transport
AU - Leifelt, Jonas
AU - Dziegiel, Morten Hanefeld
AU - Brahm, Jesper
N1 - Publisher Copyright: © 2023 Leifelt et al.
PY - 2023
Y1 - 2023
N2 - We determined the permeability (P, cm/s) of unmodified human red blood cells (HRBC) to urea (Pu), chloride (PCl), glucose (Pglu), and water diffusion (Pd) under conditions of self-exchange (SE) with the continuous flow tube method at pH 7.2, 25°C. Among 24 donors, Pu at 1 mM varied >100%. Two of the donors were also tested in 1983. Their Pu had decreased by 77 and 90%. High age in males and Kidd genotype Jk(a+,b+), but not blood types AB0, appear related to low Pu. For one of the two donors, PCl (150 mM, 38°C, pH 7.2), Pglu (1 mM, 38°C, pH 7.2), and Pd (55.5 M, 25°C, pH 7.2) were determined then and now and showed no significant changes with age. The results from six more donors show donor PCl, Pglu, and Pd in the range of ≈1%. PCl and Pglu are vital for the metabolism of cells and tissues, and we see but little donor variation, and so far, no phenotypes without glucose (GLUT1) and anion (AE1) transporters in HRBC. Phenotypes with no urea transporter (UT-B) or no water transporters (aquaporin, AQP1) are registered and are compatible with life. Our results are in line with the concept that the solutes do not share pathways in common. The great donor variation in Pu must be considered in comparative transport physiological studies.
AB - We determined the permeability (P, cm/s) of unmodified human red blood cells (HRBC) to urea (Pu), chloride (PCl), glucose (Pglu), and water diffusion (Pd) under conditions of self-exchange (SE) with the continuous flow tube method at pH 7.2, 25°C. Among 24 donors, Pu at 1 mM varied >100%. Two of the donors were also tested in 1983. Their Pu had decreased by 77 and 90%. High age in males and Kidd genotype Jk(a+,b+), but not blood types AB0, appear related to low Pu. For one of the two donors, PCl (150 mM, 38°C, pH 7.2), Pglu (1 mM, 38°C, pH 7.2), and Pd (55.5 M, 25°C, pH 7.2) were determined then and now and showed no significant changes with age. The results from six more donors show donor PCl, Pglu, and Pd in the range of ≈1%. PCl and Pglu are vital for the metabolism of cells and tissues, and we see but little donor variation, and so far, no phenotypes without glucose (GLUT1) and anion (AE1) transporters in HRBC. Phenotypes with no urea transporter (UT-B) or no water transporters (aquaporin, AQP1) are registered and are compatible with life. Our results are in line with the concept that the solutes do not share pathways in common. The great donor variation in Pu must be considered in comparative transport physiological studies.
U2 - 10.1085/jgp.202213321
DO - 10.1085/jgp.202213321
M3 - Journal article
C2 - 37535830
AN - SCOPUS:85166548405
VL - 155
JO - Journal of General Physiology
JF - Journal of General Physiology
SN - 0022-1295
IS - 10
M1 - e202213321
ER -
ID: 362331348