Mapping Cellular Coordinates through Advances in Spatial Transcriptomics Technology
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Documents
- KSMCB043-07-01
Final published version, 1.23 MB, PDF document
Complex cell-to-cell communication underlies the basic processes essential for homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach, however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.
Original language | English |
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Journal | Molecules and Cells |
Volume | 43 |
Issue number | 7 |
Pages (from-to) | 591-599 |
Number of pages | 9 |
ISSN | 1016-8478 |
DOIs | |
Publication status | Published - 2020 |
- cellular communication, single-cell RNA, spatial transcriptomics, tissue architecture, LASER CAPTURE MICRODISSECTION, IN-SITU HYBRIDIZATION, GENE-EXPRESSION, SINGLE, SEQ, TISSUE, RECONSTRUCTION, HETEROGENEITY, CONTINUUM
Research areas
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