Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids. / Parra, Boris; Cockx, Bastiaan; Lutz, Veronika T.; Brøndsted, Lone; Smets, Barth F.; Dechesne, Arnaud.

In: Microbiology Spectrum, Vol. 12, No. 1, 2024, p. e0253723.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Parra, B, Cockx, B, Lutz, VT, Brøndsted, L, Smets, BF & Dechesne, A 2024, 'Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids', Microbiology Spectrum, vol. 12, no. 1, pp. e0253723. https://doi.org/10.1128/spectrum.02537-23

APA

Parra, B., Cockx, B., Lutz, V. T., Brøndsted, L., Smets, B. F., & Dechesne, A. (2024). Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids. Microbiology Spectrum, 12(1), e0253723. https://doi.org/10.1128/spectrum.02537-23

Vancouver

Parra B, Cockx B, Lutz VT, Brøndsted L, Smets BF, Dechesne A. Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids. Microbiology Spectrum. 2024;12(1):e0253723. https://doi.org/10.1128/spectrum.02537-23

Author

Parra, Boris ; Cockx, Bastiaan ; Lutz, Veronika T. ; Brøndsted, Lone ; Smets, Barth F. ; Dechesne, Arnaud. / Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids. In: Microbiology Spectrum. 2024 ; Vol. 12, No. 1. pp. e0253723.

Bibtex

@article{cd3a62b9143a4c3fb4d51b5f39255afe,
title = "Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids",
abstract = "IMPORTANCE: This work was undertaken because plasmid-dependent phages can reduce the prevalence of conjugative plasmids and can be leveraged to prevent the acquisition and dissemination of ARGs by bacteria. The two novel phages described in this study, Lu221 and Hi226, can infect Escherichia coli, Salmonella enterica, Kluyvera sp. and Enterobacter sp. carrying conjugative plasmids. This was verified with plasmids carrying resistance determinants and belonging to the most common plasmid families among Gram-negative pathogens. Therefore, the newly isolated phages could have the potential to help control the spread of ARGs and thus help combat the antimicrobial resistance crisis.",
keywords = "antimicrobial resistance, bacteriophages, Caudoviricetes, conjugation, horizontal gene transfer, pilus, wastewater",
author = "Boris Parra and Bastiaan Cockx and Lutz, {Veronika T.} and Lone Br{\o}ndsted and Smets, {Barth F.} and Arnaud Dechesne",
year = "2024",
doi = "10.1128/spectrum.02537-23",
language = "English",
volume = "12",
pages = "e0253723",
journal = "Microbiology spectrum",
issn = "2165-0497",
publisher = "American Society for Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids

AU - Parra, Boris

AU - Cockx, Bastiaan

AU - Lutz, Veronika T.

AU - Brøndsted, Lone

AU - Smets, Barth F.

AU - Dechesne, Arnaud

PY - 2024

Y1 - 2024

N2 - IMPORTANCE: This work was undertaken because plasmid-dependent phages can reduce the prevalence of conjugative plasmids and can be leveraged to prevent the acquisition and dissemination of ARGs by bacteria. The two novel phages described in this study, Lu221 and Hi226, can infect Escherichia coli, Salmonella enterica, Kluyvera sp. and Enterobacter sp. carrying conjugative plasmids. This was verified with plasmids carrying resistance determinants and belonging to the most common plasmid families among Gram-negative pathogens. Therefore, the newly isolated phages could have the potential to help control the spread of ARGs and thus help combat the antimicrobial resistance crisis.

AB - IMPORTANCE: This work was undertaken because plasmid-dependent phages can reduce the prevalence of conjugative plasmids and can be leveraged to prevent the acquisition and dissemination of ARGs by bacteria. The two novel phages described in this study, Lu221 and Hi226, can infect Escherichia coli, Salmonella enterica, Kluyvera sp. and Enterobacter sp. carrying conjugative plasmids. This was verified with plasmids carrying resistance determinants and belonging to the most common plasmid families among Gram-negative pathogens. Therefore, the newly isolated phages could have the potential to help control the spread of ARGs and thus help combat the antimicrobial resistance crisis.

KW - antimicrobial resistance

KW - bacteriophages

KW - Caudoviricetes

KW - conjugation

KW - horizontal gene transfer

KW - pilus

KW - wastewater

U2 - 10.1128/spectrum.02537-23

DO - 10.1128/spectrum.02537-23

M3 - Journal article

C2 - 38063386

AN - SCOPUS:85182500844

VL - 12

SP - e0253723

JO - Microbiology spectrum

JF - Microbiology spectrum

SN - 2165-0497

IS - 1

ER -

ID: 380656425