HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.
Research output: Contribution to journal › Journal article › Research › peer-review
The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.
Original language | English |
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Journal | FEBS Letters |
Volume | 342 |
Issue number | 3 |
Pages (from-to) | 325-8 |
Number of pages | 3 |
ISSN | 0014-5793 |
Publication status | Published - 1994 |
Bibliographical note
Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Nucleus; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Hela Cells; Hepatocyte Nuclear Factor 1; Hepatocyte Nuclear Factor 1-alpha; Hepatocyte Nuclear Factor 1-beta; Humans; Intestinal Mucosa; Nuclear Proteins; Promoter Regions (Genetics); Protein Binding; Swine; Transcription Factors; Tumor Cells, Cultured
ID: 6586636