Healthy Nordic diet downregulates the expression of genes involved in inflammation in subcutaneous adipose tissue in individuals with features of the metabolic syndrome

Research output: Contribution to journalJournal articleResearchpeer-review

  • Marjukka Kolehmainen
  • Stine M Ulven
  • Jussi Paananen
  • Vanessa de Mello
  • Ursula Schwab
  • Carsten Carlberg
  • Mari Myhrstad
  • Jussi Pihlajamäki
  • Elisabeth Dungner
  • Eva Sjölin
  • Ingibjörg Gunnarsdottir
  • Lieselotte Cloetens
  • Mona Landin-Olsson
  • Björn Åkesson
  • Fredrik Rosqvist
  • Janne Hukkanen
  • Karl-Heinz Herzig
  • Markku J Savolainen
  • Lea Brader
  • Kjeld Hermansen
  • Ulf Risérus
  • Inga Thorsdottir
  • Kaisa S Poutanen
  • Matti Uusitupa
  • Peter Arner
  • Ingrid Dahlman

BACKGROUND: Previously, a healthy Nordic diet (ND) has been shown to have beneficial health effects close to those of Mediterranean diets.

OBJECTIVE: The objective was to explore whether the ND has an impact on gene expression in abdominal subcutaneous adipose tissue (SAT) and whether changes in gene expression are associated with clinical and biochemical effects.

DESIGN: Obese adults with features of the metabolic syndrome underwent an 18- to 24-wk randomized intervention study comparing the ND with the control diet (CD) (the SYSDIET study, carried out within Nordic Centre of Excellence of the Systems Biology in Controlled Dietary Interventions and Cohort Studies). The present study included participants from 3 Nordic SYSDIET centers [Kuopio (n = 20), Lund (n = 18), and Oulu (n = 18)] with a maximum weight change of ±4 kg, highly sensitive C-reactive protein concentration <10 mg/L at the beginning and the end of the intervention, and baseline body mass index (in kg/m(2)) <38. SAT biopsy specimens were obtained before and after the intervention and subjected to global transcriptome analysis with Gene 1.1 ST Arrays (Affymetrix).

RESULTS: Altogether, 128 genes were differentially expressed in SAT between the ND and CD (nominal P < 0.01; false discovery rate, 25%). These genes were overrepresented in pathways related to immune response (adjusted P = 0.0076), resulting mainly from slightly decreased expression in the ND and increased expression in the CD. Immune-related pathways included leukocyte trafficking and macrophage recruitment (e.g., interferon regulatory factor 1, CD97), adaptive immune response (interleukin32, interleukin 6 receptor), and reactive oxygen species (neutrophil cytosolic factor 1). Interestingly, the regulatory region of the 128 genes was overrepresented for binding sites for the nuclear transcription factor κB.

CONCLUSION: A healthy Nordic diet reduces inflammatory gene expression in SAT compared with a control diet independently of body weight change in individuals with features of the metabolic syndrome. The study was registered at as NCT00992641.

Original languageEnglish
JournalAmerican Journal of Clinical Nutrition
Issue number1
Pages (from-to)228-239
Number of pages12
Publication statusPublished - 2015

ID: 130295796