Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling. / Adhikari, Khem Bahadur; Rohde, Morten; Velschow, Sten; Feldt-Rasmussen, Ulla; Johannesen, Jesper; Johnsen, Anders Holten.

In: Bioanalysis, Vol. 12, No. 10, 05.2020, p. 665-681.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Adhikari, KB, Rohde, M, Velschow, S, Feldt-Rasmussen, U, Johannesen, J & Johnsen, AH 2020, 'Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling', Bioanalysis, vol. 12, no. 10, pp. 665-681. https://doi.org/10.4155/bio-2020-0048

APA

Adhikari, K. B., Rohde, M., Velschow, S., Feldt-Rasmussen, U., Johannesen, J., & Johnsen, A. H. (2020). Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling. Bioanalysis, 12(10), 665-681. https://doi.org/10.4155/bio-2020-0048

Vancouver

Adhikari KB, Rohde M, Velschow S, Feldt-Rasmussen U, Johannesen J, Johnsen AH. Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling. Bioanalysis. 2020 May;12(10):665-681. https://doi.org/10.4155/bio-2020-0048

Author

Adhikari, Khem Bahadur ; Rohde, Morten ; Velschow, Sten ; Feldt-Rasmussen, Ulla ; Johannesen, Jesper ; Johnsen, Anders Holten. / Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling. In: Bioanalysis. 2020 ; Vol. 12, No. 10. pp. 665-681.

Bibtex

@article{0fc89fcb3b2b49b6b7a540737bede78c,
title = "Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling",
abstract = "Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.",
author = "Adhikari, {Khem Bahadur} and Morten Rohde and Sten Velschow and Ulla Feldt-Rasmussen and Jesper Johannesen and Johnsen, {Anders Holten}",
year = "2020",
month = may,
doi = "10.4155/bio-2020-0048",
language = "English",
volume = "12",
pages = "665--681",
journal = "Bioanalysis",
issn = "1757-6180",
publisher = "Future Science",
number = "10",

}

RIS

TY - JOUR

T1 - Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling

AU - Adhikari, Khem Bahadur

AU - Rohde, Morten

AU - Velschow, Sten

AU - Feldt-Rasmussen, Ulla

AU - Johannesen, Jesper

AU - Johnsen, Anders Holten

PY - 2020/5

Y1 - 2020/5

N2 - Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.

AB - Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.

U2 - 10.4155/bio-2020-0048

DO - 10.4155/bio-2020-0048

M3 - Journal article

C2 - 32489105

VL - 12

SP - 665

EP - 681

JO - Bioanalysis

JF - Bioanalysis

SN - 1757-6180

IS - 10

ER -

ID: 250554775