Aging is associated with an altered macrophage response during human skeletal muscle regeneration

Research output: Contribution to journalJournal articleResearchpeer-review


  • Fulltext

    Final published version, 1.22 MB, PDF document

Skeletal muscle injury in aged rodents is characterized by an asynchronous infiltration of pro- and anti-inflammatory macrophage waves, leading to improper and incomplete regeneration. It is unclear whether this aberration also occurs in aged human muscle. In this study, we quantified the macrophage responses in a human model of muscle damage and regeneration induced by electrical stimulation in 7 young and 21 older adults. At baseline, total resident macrophage (CD68+/DAPI+) content was not different between young and old subjects, but pro-inflammatory (CD206−/CD68+/DAPI+) macrophage content was lower in the old. Following damage, muscle Infiltration of CD206−/CD68+/DAPI+ macrophages was lower in old relative to young subjects. Further, only the increase in CD206−/CD68+ macrophages correlated with the change in muscle satellite cell content. Our data show that older individuals have a compromised macrophage response during muscle regeneration, pointing to an altered inflammatory response as a potential mechanism for reduced muscle regenerative efficacy in aged humans.
Original languageEnglish
Article number111974
JournalExperimental Gerontology
Number of pages7
Publication statusPublished - 2022

Number of downloads are based on statistics from Google Scholar and

No data available

ID: 321945958