Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species

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The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.

TidsskriftRomanian Biotechnological Letters
Udgave nummerSUPPL.2
Sider (fra-til)56-61
Antal sider6
StatusUdgivet - 1 mar. 2010

ID: 226914862