Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species
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Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species. / Hârţa, Monica; Pamfil, Doru; Ardelean, Marin; Pedersen, Carsten; Pop, Rodica; Cordea, Mirela.
I: Romanian Biotechnological Letters, Bind 15, Nr. SUPPL.2, 01.03.2010, s. 56-61.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species
AU - Hârţa, Monica
AU - Pamfil, Doru
AU - Ardelean, Marin
AU - Pedersen, Carsten
AU - Pop, Rodica
AU - Cordea, Mirela
PY - 2010/3/1
Y1 - 2010/3/1
N2 - The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.
AB - The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.
KW - Aflp
KW - DNA polymorphism
KW - Fingerprinting techniques
KW - Ginkgo biloba
KW - Rapd
UR - http://www.scopus.com/inward/record.url?scp=78651263380&partnerID=8YFLogxK
M3 - Journal article
AN - SCOPUS:78651263380
VL - 15
SP - 56
EP - 61
JO - Romanian Biotechnological Letters
JF - Romanian Biotechnological Letters
SN - 1224-5984
IS - SUPPL.2
ER -
ID: 226914862