Impact of UV-C pretreatment on β-lactoglobulin hydrolysis by trypsin: Production and bioavailability of bioactive peptides

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  • Keila N. Cavalcante
  • Jessica F. Feitor
  • Sinara T.B. Morais
  • Renata T. Nassu
  • Ahrné, Lilia
  • Daniel R. Cardoso

The effects of UV-C light irradiation and low-temperature long-time (LTLT) pasteurization on protein structural changes, degree of hydrolysis (DH) by trypsin, peptide profile of tryptic hydrolysates by MALDI-TOF/TOF-MS, and bioavailability of β-lactoglobulin were compared. Compared with native or LTLT pasteurised samples, the hydrolysis rate constant of β-lactoglobulin treated with UV-C increased significantly, implying that the protein backbone cleavage sites became more accessible, whereas thermal treatment produced aggregates that impede trypsin activity. Tryptic hydrolyses of UV-C light treated β-lactoglobulin yielded more peptides and a more diverse peptide mass profile. Six bioactive peptides were revealed in β-LG tryptic hydrolysates of UV-C-treated protein; transepithelial transport in Caco-2 cell monolayers showed intermediate in vivo transport and absorption for three (β-LG f87–91, β-LG f91–99, and β-LG f158–164). The moderate allergen peptide LSFNPTQLEEQCHI β-LG was absent after tryptic hydrolysis of UV-C-treated protein, indicating that UV-C photolysis may be a useful tool for allergenicity reduction.

TidsskriftInternational Dairy Journal
Antal sider12
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Daniel Rodrigues Cardoso and Lilia Ahrné report financial support and equipment, drugs, or supplies were provided by State of Sao Paulo Research Foundation and Innovation Fund Denmark . Keila N. Cavalcante and Sinara T. B. Morais report master and postdoc fellowship, respectively, by Coordination of Higher Education Personnel Improvement. Daniel Rodrigues Cardoso and Renata T. Nassu report financial support was provided by National Council for Scientific and Technological Development .

Funding Information:
This research is part of the bilateral Brazilian/Danish collaboration supported by FAPESP and the Innovation Fund Denmark (FAPESP grant No. 2017/01189-0 ). KNC acknowledges CAPES for the Master-fellowship, Finance Code 001. DRC and RTN are grateful to the National Council for Scientific and Technological Development (CNPq) for the productivity research grant.

Publisher Copyright:
© 2023 Elsevier Ltd

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