Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria

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Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria. / Dougherty, Peter Erdmann; Nielsen, Tue Kjærgaard; Riber, Leise; Lading, Helen Helgå; Forero-Junco, Laura Milena; Kot, Witold; Raaijmakers, Jos M.; Hansen, Lars Hestbjerg.

I: ISME Journal, Bind 17, Nr. 12, 2023, s. 2415-2425.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Dougherty, PE, Nielsen, TK, Riber, L, Lading, HH, Forero-Junco, LM, Kot, W, Raaijmakers, JM & Hansen, LH 2023, 'Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria', ISME Journal, bind 17, nr. 12, s. 2415-2425. https://doi.org/10.1038/s41396-023-01547-1

APA

Dougherty, P. E., Nielsen, T. K., Riber, L., Lading, H. H., Forero-Junco, L. M., Kot, W., Raaijmakers, J. M., & Hansen, L. H. (2023). Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria. ISME Journal, 17(12), 2415-2425. https://doi.org/10.1038/s41396-023-01547-1

Vancouver

Dougherty PE, Nielsen TK, Riber L, Lading HH, Forero-Junco LM, Kot W o.a. Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria. ISME Journal. 2023;17(12):2415-2425. https://doi.org/10.1038/s41396-023-01547-1

Author

Dougherty, Peter Erdmann ; Nielsen, Tue Kjærgaard ; Riber, Leise ; Lading, Helen Helgå ; Forero-Junco, Laura Milena ; Kot, Witold ; Raaijmakers, Jos M. ; Hansen, Lars Hestbjerg. / Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria. I: ISME Journal. 2023 ; Bind 17, Nr. 12. s. 2415-2425.

Bibtex

@article{345dbe2a391b478e84326c3bca25ace6,

title = "Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria",

abstract = "Environmental bacteria host an enormous number of prophages, but their diversity and natural functions remain largely elusive. Here, we investigate prophage activity and diversity in 63 Erwinia and Pseudomonas strains isolated from flag leaves of wheat grown in a single field. Introducing and validating Virion Induction Profiling Sequencing (VIP-Seq), we identify and quantify the activity of 120 spontaneously induced prophages, discovering that some phyllosphere bacteria produce more than 108 virions/mL in overnight cultures, with significant induction also observed in planta. Sequence analyses and plaque assays reveal E. aphidicola prophages contribute a majority of intraspecies genetic diversity and divide their bacterial hosts into antagonistic factions engaged in widespread microbial warfare, revealing the importance of prophage-mediated microdiversity. When comparing spontaneously active prophages with predicted prophages we also find insertion sequences are strongly correlated with non-active prophages. In conclusion, we discover widespread and largely unknown prophage diversity and function in phyllosphere bacteria.",

author = "Dougherty, {Peter Erdmann} and Nielsen, {Tue Kj{\ae}rgaard} and Leise Riber and Lading, {Helen Helg{\aa}} and Forero-Junco, {Laura Milena} and Witold Kot and Raaijmakers, {Jos M.} and Hansen, {Lars Hestbjerg}",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

doi = "10.1038/s41396-023-01547-1",

language = "English",

volume = "17",

pages = "2415--2425",

journal = "I S M E Journal",

issn = "1751-7362",

publisher = "nature publishing group",

number = "12",

}

RIS

TY - JOUR

T1 - Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria

AU - Dougherty, Peter Erdmann

AU - Nielsen, Tue Kjærgaard

AU - Riber, Leise

AU - Lading, Helen Helgå

AU - Forero-Junco, Laura Milena

AU - Kot, Witold

AU - Raaijmakers, Jos M.

AU - Hansen, Lars Hestbjerg

PY - 2023

Y1 - 2023

N2 - Environmental bacteria host an enormous number of prophages, but their diversity and natural functions remain largely elusive. Here, we investigate prophage activity and diversity in 63 Erwinia and Pseudomonas strains isolated from flag leaves of wheat grown in a single field. Introducing and validating Virion Induction Profiling Sequencing (VIP-Seq), we identify and quantify the activity of 120 spontaneously induced prophages, discovering that some phyllosphere bacteria produce more than 108 virions/mL in overnight cultures, with significant induction also observed in planta. Sequence analyses and plaque assays reveal E. aphidicola prophages contribute a majority of intraspecies genetic diversity and divide their bacterial hosts into antagonistic factions engaged in widespread microbial warfare, revealing the importance of prophage-mediated microdiversity. When comparing spontaneously active prophages with predicted prophages we also find insertion sequences are strongly correlated with non-active prophages. In conclusion, we discover widespread and largely unknown prophage diversity and function in phyllosphere bacteria.

AB - Environmental bacteria host an enormous number of prophages, but their diversity and natural functions remain largely elusive. Here, we investigate prophage activity and diversity in 63 Erwinia and Pseudomonas strains isolated from flag leaves of wheat grown in a single field. Introducing and validating Virion Induction Profiling Sequencing (VIP-Seq), we identify and quantify the activity of 120 spontaneously induced prophages, discovering that some phyllosphere bacteria produce more than 108 virions/mL in overnight cultures, with significant induction also observed in planta. Sequence analyses and plaque assays reveal E. aphidicola prophages contribute a majority of intraspecies genetic diversity and divide their bacterial hosts into antagonistic factions engaged in widespread microbial warfare, revealing the importance of prophage-mediated microdiversity. When comparing spontaneously active prophages with predicted prophages we also find insertion sequences are strongly correlated with non-active prophages. In conclusion, we discover widespread and largely unknown prophage diversity and function in phyllosphere bacteria.

U2 - 10.1038/s41396-023-01547-1

DO - 10.1038/s41396-023-01547-1

M3 - Journal article

C2 - 37919394

AN - SCOPUS:85175541571

VL - 17

SP - 2415

EP - 2425

JO - I S M E Journal

JF - I S M E Journal

SN - 1751-7362

IS - 12

ER -

ID: 372823898