Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization

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Standard

Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization. / Birch, Julie Knippel Melsted; Leijon, Mikael; Nielsen, Søren Saxmose; Struve, Tina; Jensen, Henrik Elvang.

I: FEMS Microbes, Bind 2, xtab005, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Birch, JKM, Leijon, M, Nielsen, SS, Struve, T & Jensen, HE 2021, 'Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization', FEMS Microbes, bind 2, xtab005. https://doi.org/10.1093/femsmc/xtab005

APA

Birch, J. K. M., Leijon, M., Nielsen, S. S., Struve, T., & Jensen, H. E. (2021). Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization. FEMS Microbes, 2, [xtab005]. https://doi.org/10.1093/femsmc/xtab005

Vancouver

Birch JKM, Leijon M, Nielsen SS, Struve T, Jensen HE. Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization. FEMS Microbes. 2021;2. xtab005. https://doi.org/10.1093/femsmc/xtab005

Author

Birch, Julie Knippel Melsted ; Leijon, Mikael ; Nielsen, Søren Saxmose ; Struve, Tina ; Jensen, Henrik Elvang. / Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization. I: FEMS Microbes. 2021 ; Bind 2.

Bibtex

@article{509eb9fc7d9141df99d915bc4da738bc,
title = "Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization",
abstract = "Clarification of the infection microbiology remains a challenge in the pre-weaning diarrhea (PWD) syndrome in farmed mink (Neovison vison). Duodenal, jejunal and colon sections from 36 mink kits with PWD were systematically examined by chromogen in situ hybridization targeting two incriminated viruses: mink astrovirus and mink sapovirus. Using the RNAscope{\textregistered} 2.5 HD Duplex Assay, astrovirus and sapovirus were visualized and simultaneously demonstrated in the gut tissue. Both viruses infect enterocytes in the small intestine with a specific localization pattern; astrovirus affects the two apical thirds of the villi, whereas sapovirus generally affects the basal parts of the villi. Furthermore, we demonstrated that astrovirus in mink does not target the goblet cells. This is the first time astro- and calicivirus have been visualized in mink kit gut tissue, and these findings might be important in clarification of the impact of these viruses in the PWD syndrome.",
author = "Birch, {Julie Knippel Melsted} and Mikael Leijon and Nielsen, {S{\o}ren Saxmose} and Tina Struve and Jensen, {Henrik Elvang}",
year = "2021",
doi = "10.1093/femsmc/xtab005",
language = "English",
volume = "2",
journal = "FEMS Microbes",
issn = "2633-6685",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - Visualization of intestinal infections with astro- and sapovirus in mink (nevison vison) kits by in situ hybridization

AU - Birch, Julie Knippel Melsted

AU - Leijon, Mikael

AU - Nielsen, Søren Saxmose

AU - Struve, Tina

AU - Jensen, Henrik Elvang

PY - 2021

Y1 - 2021

N2 - Clarification of the infection microbiology remains a challenge in the pre-weaning diarrhea (PWD) syndrome in farmed mink (Neovison vison). Duodenal, jejunal and colon sections from 36 mink kits with PWD were systematically examined by chromogen in situ hybridization targeting two incriminated viruses: mink astrovirus and mink sapovirus. Using the RNAscope® 2.5 HD Duplex Assay, astrovirus and sapovirus were visualized and simultaneously demonstrated in the gut tissue. Both viruses infect enterocytes in the small intestine with a specific localization pattern; astrovirus affects the two apical thirds of the villi, whereas sapovirus generally affects the basal parts of the villi. Furthermore, we demonstrated that astrovirus in mink does not target the goblet cells. This is the first time astro- and calicivirus have been visualized in mink kit gut tissue, and these findings might be important in clarification of the impact of these viruses in the PWD syndrome.

AB - Clarification of the infection microbiology remains a challenge in the pre-weaning diarrhea (PWD) syndrome in farmed mink (Neovison vison). Duodenal, jejunal and colon sections from 36 mink kits with PWD were systematically examined by chromogen in situ hybridization targeting two incriminated viruses: mink astrovirus and mink sapovirus. Using the RNAscope® 2.5 HD Duplex Assay, astrovirus and sapovirus were visualized and simultaneously demonstrated in the gut tissue. Both viruses infect enterocytes in the small intestine with a specific localization pattern; astrovirus affects the two apical thirds of the villi, whereas sapovirus generally affects the basal parts of the villi. Furthermore, we demonstrated that astrovirus in mink does not target the goblet cells. This is the first time astro- and calicivirus have been visualized in mink kit gut tissue, and these findings might be important in clarification of the impact of these viruses in the PWD syndrome.

U2 - 10.1093/femsmc/xtab005

DO - 10.1093/femsmc/xtab005

M3 - Journal article

VL - 2

JO - FEMS Microbes

JF - FEMS Microbes

SN - 2633-6685

M1 - xtab005

ER -

ID: 289455746