Validation of an ELISA for detection of neutrophil gelatinase-associated lipocalin (NGAL) in equine serum
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Validation of an ELISA for detection of neutrophil gelatinase-associated lipocalin (NGAL) in equine serum. / Jacobsen, Stine; Berg, Lise C.; Tvermose, Emil; Laurberg, Malene B.; van Galen, Gaby.
I: Veterinary Clinical Pathology, Bind 47, Nr. 4, 2018, s. 603-607.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Validation of an ELISA for detection of neutrophil gelatinase-associated lipocalin (NGAL) in equine serum
AU - Jacobsen, Stine
AU - Berg, Lise C.
AU - Tvermose, Emil
AU - Laurberg, Malene B.
AU - van Galen, Gaby
PY - 2018
Y1 - 2018
N2 - Background: Neutrophil gelatinase-associated lipocalin (NGAL) has been shown to be a useful marker of kidney injury in people and dogs, but has not been described in horses. Objectives: The aim of the study was to validate a commercially available porcine-specific ELISA to measure serum concentrations of equine NGAL. Methods: Intra- and interassay imprecisions were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by the linearity under dilution. Overlapping performance was assessed by measuring NGAL concentrations in horses with normal and elevated serum creatinine levels. Results: Intra- and interassay imprecision (coefficient of variation) ranged from 5.35% to 28.39%. The ELISA showed no signs of inaccuracy. Overlapping performance was acceptable, as the assay was able to detect the expected differences of NGAL levels in horses with normal and elevated serum creatinine concentrations. Conclusions: Equine serum NGAL concentrations could be quantified reliably using the porcine-specific ELISA.
AB - Background: Neutrophil gelatinase-associated lipocalin (NGAL) has been shown to be a useful marker of kidney injury in people and dogs, but has not been described in horses. Objectives: The aim of the study was to validate a commercially available porcine-specific ELISA to measure serum concentrations of equine NGAL. Methods: Intra- and interassay imprecisions were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by the linearity under dilution. Overlapping performance was assessed by measuring NGAL concentrations in horses with normal and elevated serum creatinine levels. Results: Intra- and interassay imprecision (coefficient of variation) ranged from 5.35% to 28.39%. The ELISA showed no signs of inaccuracy. Overlapping performance was acceptable, as the assay was able to detect the expected differences of NGAL levels in horses with normal and elevated serum creatinine concentrations. Conclusions: Equine serum NGAL concentrations could be quantified reliably using the porcine-specific ELISA.
KW - Assay validation
KW - enzyme-linked immunosorbent assay
KW - horse
KW - kidney injury
KW - lipocalin 2
KW - neutrophil gelatinase-associated lipocalin
U2 - 10.1111/vcp.12670
DO - 10.1111/vcp.12670
M3 - Journal article
C2 - 30403420
AN - SCOPUS:85056163569
VL - 47
SP - 603
EP - 607
JO - Veterinary Clinical Pathology
JF - Veterinary Clinical Pathology
SN - 0275-6382
IS - 4
ER -
ID: 210066755