Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Standard

Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections. / Alfaro Nuñez, Luis Alonso; Gilbert, M Thomas P.

I: Journal of Virological Methods, Bind 206, 09.2014, s. 38-41.

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Harvard

Alfaro Nuñez, LA & Gilbert, MTP 2014, 'Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections', Journal of Virological Methods, bind 206, s. 38-41. https://doi.org/10.1016/j.jviromet.2014.05.019

APA

Alfaro Nuñez, L. A., & Gilbert, M. T. P. (2014). Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections. Journal of Virological Methods, 206, 38-41. https://doi.org/10.1016/j.jviromet.2014.05.019

Vancouver

Alfaro Nuñez LA, Gilbert MTP. Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections. Journal of Virological Methods. 2014 sep.;206:38-41. https://doi.org/10.1016/j.jviromet.2014.05.019

Author

Alfaro Nuñez, Luis Alonso ; Gilbert, M Thomas P. / Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections. I: Journal of Virological Methods. 2014 ; Bind 206. s. 38-41.

Bibtex

@article{0d4e152e222e428d8cc76f1522bca52c,

title = "Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections",

abstract = "The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles.",

author = "{Alfaro Nu{\~n}ez}, {Luis Alonso} and Gilbert, {M Thomas P}",

year = "2014",

month = sep,

doi = "10.1016/j.jviromet.2014.05.019",

language = "English",

volume = "206",

pages = "38--41",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections

AU - Alfaro Nuñez, Luis Alonso

AU - Gilbert, M Thomas P

PY - 2014/9

Y1 - 2014/9

N2 - The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles.

AB - The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles.

U2 - 10.1016/j.jviromet.2014.05.019

DO - 10.1016/j.jviromet.2014.05.019

M3 - Letter

C2 - 24882497

VL - 206

SP - 38

EP - 41

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

ER -

ID: 129542618