Uncovering new substrates for Aurora A kinase

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Standard

Uncovering new substrates for Aurora A kinase. / Sardon, Teresa; Pache, Roland A; Stein, Amelie; Molina, Henrik; Vernos, Isabelle; Aloy, Patrick.

I: EMBO Reports, Bind 11, Nr. 12, 2010, s. 977-984.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Sardon, T, Pache, RA, Stein, A, Molina, H, Vernos, I & Aloy, P 2010, 'Uncovering new substrates for Aurora A kinase', EMBO Reports, bind 11, nr. 12, s. 977-984. https://doi.org/10.1038/embor.2010.171

APA

Sardon, T., Pache, R. A., Stein, A., Molina, H., Vernos, I., & Aloy, P. (2010). Uncovering new substrates for Aurora A kinase. EMBO Reports, 11(12), 977-984. https://doi.org/10.1038/embor.2010.171

Vancouver

Sardon T, Pache RA, Stein A, Molina H, Vernos I, Aloy P. Uncovering new substrates for Aurora A kinase. EMBO Reports. 2010;11(12):977-984. https://doi.org/10.1038/embor.2010.171

Author

Sardon, Teresa ; Pache, Roland A ; Stein, Amelie ; Molina, Henrik ; Vernos, Isabelle ; Aloy, Patrick. / Uncovering new substrates for Aurora A kinase. I: EMBO Reports. 2010 ; Bind 11, Nr. 12. s. 977-984.

Bibtex

@article{b03f5a95785847b2af34a466eea04109,
title = "Uncovering new substrates for Aurora A kinase",
abstract = "Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.",
keywords = "Amino Acid Sequence, Aurora Kinases, Computational Biology, Humans, Microtubule-Associated Proteins/chemistry, Mitosis, Models, Biological, Molecular Sequence Data, Phosphorylation, Phosphoserine/metabolism, Protein-Serine-Threonine Kinases/metabolism, Reproducibility of Results, Substrate Specificity",
author = "Teresa Sardon and Pache, {Roland A} and Amelie Stein and Henrik Molina and Isabelle Vernos and Patrick Aloy",
year = "2010",
doi = "10.1038/embor.2010.171",
language = "English",
volume = "11",
pages = "977--984",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",
number = "12",

}

RIS

TY - JOUR

T1 - Uncovering new substrates for Aurora A kinase

AU - Sardon, Teresa

AU - Pache, Roland A

AU - Stein, Amelie

AU - Molina, Henrik

AU - Vernos, Isabelle

AU - Aloy, Patrick

PY - 2010

Y1 - 2010

N2 - Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.

AB - Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.

KW - Amino Acid Sequence

KW - Aurora Kinases

KW - Computational Biology

KW - Humans

KW - Microtubule-Associated Proteins/chemistry

KW - Mitosis

KW - Models, Biological

KW - Molecular Sequence Data

KW - Phosphorylation

KW - Phosphoserine/metabolism

KW - Protein-Serine-Threonine Kinases/metabolism

KW - Reproducibility of Results

KW - Substrate Specificity

U2 - 10.1038/embor.2010.171

DO - 10.1038/embor.2010.171

M3 - Journal article

C2 - 21072059

VL - 11

SP - 977

EP - 984

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 12

ER -

ID: 203256455