Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO

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Standard

Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO. / Iba, K; Sawada, N; Chiba, H; Wewer, U M; Ishii, S; Mori, M.

I: F E B S Letters, Bind 373, Nr. 1, 02.10.1995, s. 1-4.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Iba, K, Sawada, N, Chiba, H, Wewer, UM, Ishii, S & Mori, M 1995, 'Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO', F E B S Letters, bind 373, nr. 1, s. 1-4.

APA

Iba, K., Sawada, N., Chiba, H., Wewer, U. M., Ishii, S., & Mori, M. (1995). Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO. F E B S Letters, 373(1), 1-4.

Vancouver

Iba K, Sawada N, Chiba H, Wewer UM, Ishii S, Mori M. Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO. F E B S Letters. 1995 okt. 2;373(1):1-4.

Author

Iba, K ; Sawada, N ; Chiba, H ; Wewer, U M ; Ishii, S ; Mori, M. / Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO. I: F E B S Letters. 1995 ; Bind 373, Nr. 1. s. 1-4.

Bibtex

@article{358c1da2398b449e8a7b781c34c3ab32,
title = "Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO",
abstract = "In the present study, we examined the regulation of tetranectin gene expression using a human osteoblastic cell line, SV-HFO, that undergoes mineralization upon treatment with dexamethasone. We found that the expression of tetranectin and alkaline phosphatase mRNA was induced by dexamethasone treatment as evidenced by Northern blotting. When transforming growth factor-beta 1 (TGF-beta 1) was added together with dexamethasone to the SV-HFO cell cultures, the mineralization process was markedly suppressed and the expression of tetra nectin and alkaline phosphatase was downregulated in a dose-dependent manner. These results demonstrate that the expression of tetranectin in these osteoblastic cells is regulated by dexamethasone and TGF-beta 1 and that tetranectin expression is tightly linked to the process of mineralization.",
keywords = "Blood Proteins, Blotting, Northern, Cell Line, Dexamethasone, Gene Expression, Humans, Lectins, C-Type, Osteoblasts, RNA, Messenger, Transforming Growth Factor beta, Tumor Markers, Biological",
author = "K Iba and N Sawada and H Chiba and Wewer, {U M} and S Ishii and M Mori",
year = "1995",
month = oct,
day = "2",
language = "English",
volume = "373",
pages = "1--4",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Transforming growth factor-beta 1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO

AU - Iba, K

AU - Sawada, N

AU - Chiba, H

AU - Wewer, U M

AU - Ishii, S

AU - Mori, M

PY - 1995/10/2

Y1 - 1995/10/2

N2 - In the present study, we examined the regulation of tetranectin gene expression using a human osteoblastic cell line, SV-HFO, that undergoes mineralization upon treatment with dexamethasone. We found that the expression of tetranectin and alkaline phosphatase mRNA was induced by dexamethasone treatment as evidenced by Northern blotting. When transforming growth factor-beta 1 (TGF-beta 1) was added together with dexamethasone to the SV-HFO cell cultures, the mineralization process was markedly suppressed and the expression of tetra nectin and alkaline phosphatase was downregulated in a dose-dependent manner. These results demonstrate that the expression of tetranectin in these osteoblastic cells is regulated by dexamethasone and TGF-beta 1 and that tetranectin expression is tightly linked to the process of mineralization.

AB - In the present study, we examined the regulation of tetranectin gene expression using a human osteoblastic cell line, SV-HFO, that undergoes mineralization upon treatment with dexamethasone. We found that the expression of tetranectin and alkaline phosphatase mRNA was induced by dexamethasone treatment as evidenced by Northern blotting. When transforming growth factor-beta 1 (TGF-beta 1) was added together with dexamethasone to the SV-HFO cell cultures, the mineralization process was markedly suppressed and the expression of tetra nectin and alkaline phosphatase was downregulated in a dose-dependent manner. These results demonstrate that the expression of tetranectin in these osteoblastic cells is regulated by dexamethasone and TGF-beta 1 and that tetranectin expression is tightly linked to the process of mineralization.

KW - Blood Proteins

KW - Blotting, Northern

KW - Cell Line

KW - Dexamethasone

KW - Gene Expression

KW - Humans

KW - Lectins, C-Type

KW - Osteoblasts

KW - RNA, Messenger

KW - Transforming Growth Factor beta

KW - Tumor Markers, Biological

M3 - Journal article

C2 - 7589422

VL - 373

SP - 1

EP - 4

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1

ER -

ID: 34326091