Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions

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Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions. / Morgani, Sophie M; Canham, Maurice A; Nichols, Jennifer; Sharov, Alexei A; Migueles, Rosa Portero; Ko, Minoru S H; Brickman, Joshua M.

I: Cell Reports, 04.06.2013, s. 1945–1957.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Morgani, SM, Canham, MA, Nichols, J, Sharov, AA, Migueles, RP, Ko, MSH & Brickman, JM 2013, 'Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions', Cell Reports, s. 1945–1957. https://doi.org/10.1016/j.celrep.2013.04.034

APA

Morgani, S. M., Canham, M. A., Nichols, J., Sharov, A. A., Migueles, R. P., Ko, M. S. H., & Brickman, J. M. (2013). Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions. Cell Reports, 1945–1957. https://doi.org/10.1016/j.celrep.2013.04.034

Vancouver

Morgani SM, Canham MA, Nichols J, Sharov AA, Migueles RP, Ko MSH o.a. Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions. Cell Reports. 2013 jun. 4;1945–1957. https://doi.org/10.1016/j.celrep.2013.04.034

Author

Morgani, Sophie M ; Canham, Maurice A ; Nichols, Jennifer ; Sharov, Alexei A ; Migueles, Rosa Portero ; Ko, Minoru S H ; Brickman, Joshua M. / Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions. I: Cell Reports. 2013 ; s. 1945–1957.

Bibtex

@article{329b4792fe804ddfb29039cde24e4e45,

title = "Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions",

abstract = "Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition from totipotency, when individual blastomeres can make all lineages, to pluripotency, when they are competent to make only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) are thought to represent an embryonically restricted ground state. However, we observed heterogeneous expression of the extraembryonic endoderm marker Hex in 2i-cultured embryos, suggesting that 2i blocked development prior to epiblast commitment. Similarly, 2i ESC cultures were heterogeneous and contained a Hex-positive fraction primed to differentiate into trophoblast and extraembryonic endoderm. Single Hex-positive ESCs coexpressed epiblast and extraembryonic genes and contributed to all lineages in chimeras. The cytokine LIF, necessary for ESC self-renewal, supported the expansion of this population but did not directly support Nanog-positive epiblast-like ESCs. Thus, 2i and LIF support a totipotent state comparable to early embryonic cells that coexpress embryonic and extraembryonic determinants.",

author = "Morgani, {Sophie M} and Canham, {Maurice A} and Jennifer Nichols and Sharov, {Alexei A} and Migueles, {Rosa Portero} and Ko, {Minoru S H} and Brickman, {Joshua M}",

year = "2013",

month = jun,

day = "4",

doi = "10.1016/j.celrep.2013.04.034",

language = "English",

pages = "1945–1957",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

}

RIS

TY - JOUR

T1 - Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions

AU - Morgani, Sophie M

AU - Canham, Maurice A

AU - Nichols, Jennifer

AU - Sharov, Alexei A

AU - Migueles, Rosa Portero

AU - Ko, Minoru S H

AU - Brickman, Joshua M

PY - 2013/6/4

Y1 - 2013/6/4

N2 - Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition from totipotency, when individual blastomeres can make all lineages, to pluripotency, when they are competent to make only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) are thought to represent an embryonically restricted ground state. However, we observed heterogeneous expression of the extraembryonic endoderm marker Hex in 2i-cultured embryos, suggesting that 2i blocked development prior to epiblast commitment. Similarly, 2i ESC cultures were heterogeneous and contained a Hex-positive fraction primed to differentiate into trophoblast and extraembryonic endoderm. Single Hex-positive ESCs coexpressed epiblast and extraembryonic genes and contributed to all lineages in chimeras. The cytokine LIF, necessary for ESC self-renewal, supported the expansion of this population but did not directly support Nanog-positive epiblast-like ESCs. Thus, 2i and LIF support a totipotent state comparable to early embryonic cells that coexpress embryonic and extraembryonic determinants.

AB - Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition from totipotency, when individual blastomeres can make all lineages, to pluripotency, when they are competent to make only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) are thought to represent an embryonically restricted ground state. However, we observed heterogeneous expression of the extraembryonic endoderm marker Hex in 2i-cultured embryos, suggesting that 2i blocked development prior to epiblast commitment. Similarly, 2i ESC cultures were heterogeneous and contained a Hex-positive fraction primed to differentiate into trophoblast and extraembryonic endoderm. Single Hex-positive ESCs coexpressed epiblast and extraembryonic genes and contributed to all lineages in chimeras. The cytokine LIF, necessary for ESC self-renewal, supported the expansion of this population but did not directly support Nanog-positive epiblast-like ESCs. Thus, 2i and LIF support a totipotent state comparable to early embryonic cells that coexpress embryonic and extraembryonic determinants.

U2 - 10.1016/j.celrep.2013.04.034

DO - 10.1016/j.celrep.2013.04.034

M3 - Journal article

C2 - 23746443

SP - 1945

EP - 1957

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

ER -

ID: 46259647