Total RNA sequencing reveals multilevel microbial community changes and functional responses to wood ash application in agricultural and forest soil
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Total RNA sequencing reveals multilevel microbial community changes and functional responses to wood ash application in agricultural and forest soil. / Bang-Andreasen, Toke; Anwar, Muhammad Zohaib; Lanzén, Anders; Kjøller, Rasmus; Rønn, Regin; Ekelund, Flemming; Jacobsen, Carsten Suhr.
I: FEMS Microbiology Ecology, Bind 96, Nr. 3, fiaa016, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Total RNA sequencing reveals multilevel microbial community changes and functional responses to wood ash application in agricultural and forest soil
AU - Bang-Andreasen, Toke
AU - Anwar, Muhammad Zohaib
AU - Lanzén, Anders
AU - Kjøller, Rasmus
AU - Rønn, Regin
AU - Ekelund, Flemming
AU - Jacobsen, Carsten Suhr
PY - 2020
Y1 - 2020
N2 - Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied total RNA sequencing to simultaneously assess the impact of wood ash amendment on the active soil microbial communities and the expression of functional genes from all microbial taxa. Wood ash significantly affected the taxonomic (rRNA) as well as functional (mRNA) profiles of both agricultural and forest soil. Increase in pH, electrical conductivity, dissolved organic carbon and phosphate were the most important physicochemical drivers for the observed changes. Wood ash amendment increased the relative abundance of the copiotrophic groups Chitinonophagaceae (Bacteroidetes) and Rhizobiales (Alphaproteobacteria) and resulted in higher expression of genes involved in metabolism and cell growth. Finally, total RNA sequencing allowed us to show that some groups of bacterial feeding protozoa increased concomitantly to the enhanced bacterial growth, which shows their pivotal role in the regulation of bacterial abundance in soil.
AB - Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied total RNA sequencing to simultaneously assess the impact of wood ash amendment on the active soil microbial communities and the expression of functional genes from all microbial taxa. Wood ash significantly affected the taxonomic (rRNA) as well as functional (mRNA) profiles of both agricultural and forest soil. Increase in pH, electrical conductivity, dissolved organic carbon and phosphate were the most important physicochemical drivers for the observed changes. Wood ash amendment increased the relative abundance of the copiotrophic groups Chitinonophagaceae (Bacteroidetes) and Rhizobiales (Alphaproteobacteria) and resulted in higher expression of genes involved in metabolism and cell growth. Finally, total RNA sequencing allowed us to show that some groups of bacterial feeding protozoa increased concomitantly to the enhanced bacterial growth, which shows their pivotal role in the regulation of bacterial abundance in soil.
KW - biodiversity
KW - metatranscriptomics
KW - protozoa
KW - soil biota
KW - total RNA
KW - wood ash
U2 - 10.1093/femsec/fiaa016
DO - 10.1093/femsec/fiaa016
M3 - Journal article
C2 - 32009159
AN - SCOPUS:85080845536
VL - 96
JO - F E M S Microbiology Ecology
JF - F E M S Microbiology Ecology
SN - 0168-6496
IS - 3
M1 - fiaa016
ER -
ID: 238425270