The role of the interleukin-10 subfamily members in immunoglobulin production by human B cells
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The role of the interleukin-10 subfamily members in immunoglobulin production by human B cells. / Hummelshoj, L; Ryder, L P; Poulsen, Lars K.
I: Scandinavian Journal of Immunology, Bind 64, Nr. 1, 2006, s. 40-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The role of the interleukin-10 subfamily members in immunoglobulin production by human B cells
AU - Hummelshoj, L
AU - Ryder, L P
AU - Poulsen, Lars K.
PY - 2006
Y1 - 2006
N2 - Interleukin (IL)-10 has been shown to have various effects on B cells, including positively affecting the production of immunoglobulin A (IgA) and IgG. Several human IL-10-related molecules have been identified. These include IL-19, IL-20, IL-22, IL-24, IL-26, IL-28 and IL-29. To determine the effects of the IL-10 analogues on the class switch recombination in B cells, we analysed Ig production from naïve B cells stimulated with these cytokines in the presence of anti-CD40. None of the cytokines were found to induce Ig production by themselves in the presence of anti-CD40 Ab. However, all cytokines inhibited the production of IgA and IgG induced by anti-CD40 Ab alone. In combination with anti-CD40 Ab and IL-4, IgG4 were inhibited in cultures stimulated with IL-20, IL-22, IL-26, IL-28 and IL-29 compared with IL-4 and anti-CD40 Ab alone, whereas all IL-10 analogues increased the production of total IgG. All analogues reduced anti-CD40 Ab + transforming growth factor (TGF)-beta-induced IgA production compared with cultures stimulated with anti-CD40 Ab and TGF-beta alone. Together, these data show that the IL-10-related cytokines in combination with anti-CD40 Ab are not by themselves directly involved in the Ig regulation in B cells. However, some of the analogues might have regulatory effects on CSR induced by CD40-ligation in combination with IL-4 or TGF-beta.
AB - Interleukin (IL)-10 has been shown to have various effects on B cells, including positively affecting the production of immunoglobulin A (IgA) and IgG. Several human IL-10-related molecules have been identified. These include IL-19, IL-20, IL-22, IL-24, IL-26, IL-28 and IL-29. To determine the effects of the IL-10 analogues on the class switch recombination in B cells, we analysed Ig production from naïve B cells stimulated with these cytokines in the presence of anti-CD40. None of the cytokines were found to induce Ig production by themselves in the presence of anti-CD40 Ab. However, all cytokines inhibited the production of IgA and IgG induced by anti-CD40 Ab alone. In combination with anti-CD40 Ab and IL-4, IgG4 were inhibited in cultures stimulated with IL-20, IL-22, IL-26, IL-28 and IL-29 compared with IL-4 and anti-CD40 Ab alone, whereas all IL-10 analogues increased the production of total IgG. All analogues reduced anti-CD40 Ab + transforming growth factor (TGF)-beta-induced IgA production compared with cultures stimulated with anti-CD40 Ab and TGF-beta alone. Together, these data show that the IL-10-related cytokines in combination with anti-CD40 Ab are not by themselves directly involved in the Ig regulation in B cells. However, some of the analogues might have regulatory effects on CSR induced by CD40-ligation in combination with IL-4 or TGF-beta.
KW - Animals
KW - Antigens, CD40
KW - B-Lymphocytes
KW - Cell Line
KW - Cytidine Deaminase
KW - Genes, Immunoglobulin
KW - Humans
KW - Immunoglobulin A
KW - Immunoglobulin Class Switching
KW - Immunoglobulin E
KW - Immunoglobulin G
KW - Interleukin-10
KW - Interleukins
KW - Mice
KW - Transcription, Genetic
KW - Transforming Growth Factor beta
U2 - 10.1111/j.1365-3083.2006.01773.x
DO - 10.1111/j.1365-3083.2006.01773.x
M3 - Journal article
C2 - 16784489
VL - 64
SP - 40
EP - 47
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 1
ER -
ID: 43535657