The location of octenyl succinate anhydride groups in high-amylose maize starch granules and its effect on stability of pickering emulsion stability
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Octenyl succinate anhydride (OSA) modified high amylose maize starch (HAMS) showed limited emulsion stability due to the OSA groups were located on the surface of starch granules. However, amylose is enriched in the internal region of HAMS and provides the reaction site for OSA groups. In this study, five types of starches with different amylose content were prepared using optimized reaction conditions. Our data showed that amylose content (AC) was the most important parameter controlling the degree of substitution (DS), in comparison with temperature, pH, starch concentration and time. DS reached a maximum (2.1%) at AC of 58%. Importantly, OSA groups were mainly located in the internal regions of HAMS under the optimized reaction conditions. This was supported by (1) the decrease of the crystallinity, (2) the weaker fluorescence intensity of the starch fluorophore probe 8-amino-1,3,6-pyrenetrisulfonic acid (APTS) in the internal regions of HAMS, and (3) unchanged signals from Fourier transform infrared spectroscopy which characterized the granular surface structure ordering. This new OSA reaction pattern permits HAMS to be used in stabilizing Pickering emulsions This is the first report that OSA groups located in the internal regions of HAMS granules.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 113892 |
| Tidsskrift | LWT |
| Vol/bind | 169 |
| Antal sider | 11 |
| ISSN | 0023-6438 |
| DOI | |
| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
The synthesis process of OSA starch was optimized by an orthogonal test. At 58%, amylose content, starch concentration of 35%, temperature at 35 °C, pH at 8.0, and 5 h reaction time, the product gained the highest DS; 2.1%, and showed a more internal distribution of OSA groups as compared with other protocols. The order of influence was: amylose content > temperature > pH > starch concentration > time. OSA treatment of maize starches mainly decreased the crystallinity and fluorescence intensity of APTS in the internal part of granules, suggesting that OSA groups existed internally in the starch granules, which supports our hypothesis. Compared with MNMS, MHAMS had higher DS and higher amounts of OSA groups in the internal regions of granules. As compared to MNMS, MHAMS-based emulsions showed a greater effect on preventing the coalescence of oil droplets and enhancing emulsion stability. This is possibly due to that more OSA groups in the internal regions of MHAMS granules contributed to its greater ability to accommodating molecules in the internal regions of granules, thereby preventing the coalescence between oil droplets by volume exclusion and steric hindrance. Overall, the results showed that OSA modified high amylose starch synthesized by an orthogonal test had the ability to generate optimized stabilized Pickering emulsions.This work was financially supported by 2021 Agricultural Technology and Innovation Program (NYKJ-2021-YL(XN)13) of Department of Agriculture and Rural Affairs of Shaanxi Province, China, Special corn germplasm creation, Variety Breeding and Industrialization Demonstration (2021ZDLNY01-08) and Construction of Agricultural Science and Technology Demonstration Park in Kazakhstan (Z2220121005).
Funding Information:
This work was financially supported by 2021 Agricultural Technology and Innovation Program ( NYKJ-2021-YL(XN)13 ) of Department of Agriculture and Rural Affairs of Shaanxi Province, China, Special corn germplasm creation, Variety Breeding and Industrialization Demonstration ( 2021ZDLNY01-08 ) and Construction of Agricultural Science and Technology Demonstration Park in Kazakhstan ( Z2220121005 ).
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© 2022
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