The free radical spin-trap alpha-PBN attenuates periinfarct depolarizations following permanent middle cerebral artery occlusion in rats without reducing infarct volume
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The free radical spin-trap alpha-PBN attenuates periinfarct depolarizations following permanent middle cerebral artery occlusion in rats without reducing infarct volume. / Christensen, Thomas; Bruhn, Torben; Diemer, Nils Henrik.
I: Brain Research, Bind 990, Nr. 1-2, 14.11.2003, s. 66-76.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The free radical spin-trap alpha-PBN attenuates periinfarct depolarizations following permanent middle cerebral artery occlusion in rats without reducing infarct volume
AU - Christensen, Thomas
AU - Bruhn, Torben
AU - Diemer, Nils Henrik
PY - 2003/11/14
Y1 - 2003/11/14
N2 - The effect of the free radical spin-trap alpha-phenyl-butyl-tert-nitrone (alpha-PBN) in permanent focal cerebral ischemia in rats was examined in two series of experiments. In the first, rats were subjected to permanent occlusion of the middle cerebral artery (MCAO) and treated 1 h after occlusion with a single dose of alpha-PBN (100 mg/kg) or saline. Body temperature was measured and controlled for the first 24 h to obtain identical temperature curves in the two groups. Cortical infarct volumes were determined on histological sections 7 days later. alpha-PBN did not significantly reduce infarct volume (control: 28.3+/-16.3 mm3 vs. alpha-PBN 23.7+/-7.4 mm3). In the second series of experiments, periinfarct depolarizations (PIDs) were recorded with an extracellular DC electrode at two locations in the ischemic penumbra for the initial 3 h following MCAO. alpha-PBN (100 mg/kg, single dose in conjunction with occlusion) significantly reduced the total number (median value of 3 PIDs in the control groups vs. 1 PID in alpha-PBN groups, p
AB - The effect of the free radical spin-trap alpha-phenyl-butyl-tert-nitrone (alpha-PBN) in permanent focal cerebral ischemia in rats was examined in two series of experiments. In the first, rats were subjected to permanent occlusion of the middle cerebral artery (MCAO) and treated 1 h after occlusion with a single dose of alpha-PBN (100 mg/kg) or saline. Body temperature was measured and controlled for the first 24 h to obtain identical temperature curves in the two groups. Cortical infarct volumes were determined on histological sections 7 days later. alpha-PBN did not significantly reduce infarct volume (control: 28.3+/-16.3 mm3 vs. alpha-PBN 23.7+/-7.4 mm3). In the second series of experiments, periinfarct depolarizations (PIDs) were recorded with an extracellular DC electrode at two locations in the ischemic penumbra for the initial 3 h following MCAO. alpha-PBN (100 mg/kg, single dose in conjunction with occlusion) significantly reduced the total number (median value of 3 PIDs in the control groups vs. 1 PID in alpha-PBN groups, p
KW - Animals
KW - Body Temperature
KW - Brain Ischemia
KW - Cerebrovascular Circulation
KW - Cortical Spreading Depression
KW - Cyclic N-Oxides
KW - Electrophysiology
KW - Extracellular Space
KW - Free Radical Scavengers
KW - Free Radicals
KW - Glial Fibrillary Acidic Protein
KW - Infarction, Middle Cerebral Artery
KW - Laser-Doppler Flowmetry
KW - Male
KW - Nitrogen Oxides
KW - Potassium Chloride
KW - Rats
KW - Rats, Wistar
KW - Survival
KW - Time Factors
M3 - Journal article
C2 - 14568331
VL - 990
SP - 66
EP - 76
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -
ID: 45392266