The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa

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Standard

The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa. / Broniewski, Jenny M; Chisnall, Matthew A W; Høyland-Kroghsbo, Nina Molin; Buckling, Angus; Westra, Edze R.

I: I S M E Journal, Bind 15, 2021, s. 2465-2473.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Broniewski, JM, Chisnall, MAW, Høyland-Kroghsbo, NM, Buckling, A & Westra, ER 2021, 'The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa', I S M E Journal, bind 15, s. 2465-2473. https://doi.org/10.1038/s41396-021-00946-6

APA

Broniewski, J. M., Chisnall, M. A. W., Høyland-Kroghsbo, N. M., Buckling, A., & Westra, E. R. (2021). The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa. I S M E Journal, 15, 2465-2473. https://doi.org/10.1038/s41396-021-00946-6

Vancouver

Broniewski JM, Chisnall MAW, Høyland-Kroghsbo NM, Buckling A, Westra ER. The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa. I S M E Journal. 2021;15:2465-2473. https://doi.org/10.1038/s41396-021-00946-6

Author

Broniewski, Jenny M ; Chisnall, Matthew A W ; Høyland-Kroghsbo, Nina Molin ; Buckling, Angus ; Westra, Edze R. / The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa. I: I S M E Journal. 2021 ; Bind 15. s. 2465-2473.

Bibtex

@article{caff0689fb9f447b8059673c2de4ceea,
title = "The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa",
abstract = "Quorum sensing controls the expression of a wide range of important traits in the opportunistic pathogen Pseudomonas aeruginosa, including the expression of virulence genes and its CRISPR-cas immune system, which protects from bacteriophage (phage) infection. This finding has led to the speculation that synthetic quorum sensing inhibitors could be used to limit the evolution of CRISPR immunity during phage therapy. Here we use experimental evolution to explore if and how a quorum sensing inhibitor influences the population and evolutionary dynamics of P. aeruginosa upon phage DMS3vir infection. We find that chemical inhibition of quorum sensing decreases phage adsorption rates due to downregulation of the Type IV pilus, which causes delayed lysis of bacterial cultures and favours the evolution of CRISPR immunity. Our data therefore suggest that inhibiting quorum sensing may reduce rather than improve the therapeutic efficacy of pilus-specific phage, and this is likely a general feature when phage receptors are positively regulated by quorum sensing.",
author = "Broniewski, {Jenny M} and Chisnall, {Matthew A W} and H{\o}yland-Kroghsbo, {Nina Molin} and Angus Buckling and Westra, {Edze R}",
year = "2021",
doi = "10.1038/s41396-021-00946-6",
language = "English",
volume = "15",
pages = "2465--2473",
journal = "I S M E Journal",
issn = "1751-7362",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The effect of Quorum sensing inhibitors on the evolution of CRISPR-based phage immunity in Pseudomonas aeruginosa

AU - Broniewski, Jenny M

AU - Chisnall, Matthew A W

AU - Høyland-Kroghsbo, Nina Molin

AU - Buckling, Angus

AU - Westra, Edze R

PY - 2021

Y1 - 2021

N2 - Quorum sensing controls the expression of a wide range of important traits in the opportunistic pathogen Pseudomonas aeruginosa, including the expression of virulence genes and its CRISPR-cas immune system, which protects from bacteriophage (phage) infection. This finding has led to the speculation that synthetic quorum sensing inhibitors could be used to limit the evolution of CRISPR immunity during phage therapy. Here we use experimental evolution to explore if and how a quorum sensing inhibitor influences the population and evolutionary dynamics of P. aeruginosa upon phage DMS3vir infection. We find that chemical inhibition of quorum sensing decreases phage adsorption rates due to downregulation of the Type IV pilus, which causes delayed lysis of bacterial cultures and favours the evolution of CRISPR immunity. Our data therefore suggest that inhibiting quorum sensing may reduce rather than improve the therapeutic efficacy of pilus-specific phage, and this is likely a general feature when phage receptors are positively regulated by quorum sensing.

AB - Quorum sensing controls the expression of a wide range of important traits in the opportunistic pathogen Pseudomonas aeruginosa, including the expression of virulence genes and its CRISPR-cas immune system, which protects from bacteriophage (phage) infection. This finding has led to the speculation that synthetic quorum sensing inhibitors could be used to limit the evolution of CRISPR immunity during phage therapy. Here we use experimental evolution to explore if and how a quorum sensing inhibitor influences the population and evolutionary dynamics of P. aeruginosa upon phage DMS3vir infection. We find that chemical inhibition of quorum sensing decreases phage adsorption rates due to downregulation of the Type IV pilus, which causes delayed lysis of bacterial cultures and favours the evolution of CRISPR immunity. Our data therefore suggest that inhibiting quorum sensing may reduce rather than improve the therapeutic efficacy of pilus-specific phage, and this is likely a general feature when phage receptors are positively regulated by quorum sensing.

U2 - 10.1038/s41396-021-00946-6

DO - 10.1038/s41396-021-00946-6

M3 - Journal article

C2 - 33692485

VL - 15

SP - 2465

EP - 2473

JO - I S M E Journal

JF - I S M E Journal

SN - 1751-7362

ER -

ID: 258184907