Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations

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Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations. / Chen, Ya-Wen; Song, Shilin; Weng, Ruifen; Verma, Pushpa; Kugler, Jan-Michael; Buescher, Marita; Rouam, Sigrid; Cohen, Stephen M.

I: Developmental Cell, Bind 31, Nr. 6, 22.12.2014, s. 784-800.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Chen, Y-W, Song, S, Weng, R, Verma, P, Kugler, J-M, Buescher, M, Rouam, S & Cohen, SM 2014, 'Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations', Developmental Cell, bind 31, nr. 6, s. 784-800. https://doi.org/10.1016/j.devcel.2014.11.029

APA

Chen, Y-W., Song, S., Weng, R., Verma, P., Kugler, J-M., Buescher, M., Rouam, S., & Cohen, S. M. (2014). Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations. Developmental Cell, 31(6), 784-800. https://doi.org/10.1016/j.devcel.2014.11.029

Vancouver

Chen Y-W, Song S, Weng R, Verma P, Kugler J-M, Buescher M o.a. Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations. Developmental Cell. 2014 dec. 22;31(6):784-800. https://doi.org/10.1016/j.devcel.2014.11.029

Author

Chen, Ya-Wen ; Song, Shilin ; Weng, Ruifen ; Verma, Pushpa ; Kugler, Jan-Michael ; Buescher, Marita ; Rouam, Sigrid ; Cohen, Stephen M. / Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations. I: Developmental Cell. 2014 ; Bind 31, Nr. 6. s. 784-800.

Bibtex

@article{1f31471c3eca4121bf112c4a1bb63020,

title = "Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations",

abstract = "MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.",

author = "Ya-Wen Chen and Shilin Song and Ruifen Weng and Pushpa Verma and Jan-Michael Kugler and Marita Buescher and Sigrid Rouam and Cohen, {Stephen M}",

year = "2014",

month = dec,

day = "22",

doi = "10.1016/j.devcel.2014.11.029",

language = "English",

volume = "31",

pages = "784--800",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "6",

}

RIS

TY - JOUR

T1 - Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations

AU - Chen, Ya-Wen

AU - Song, Shilin

AU - Weng, Ruifen

AU - Verma, Pushpa

AU - Kugler, Jan-Michael

AU - Buescher, Marita

AU - Rouam, Sigrid

AU - Cohen, Stephen M

PY - 2014/12/22

Y1 - 2014/12/22

N2 - MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

AB - MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

U2 - 10.1016/j.devcel.2014.11.029

DO - 10.1016/j.devcel.2014.11.029

M3 - Journal article

C2 - 25535920

VL - 31

SP - 784

EP - 800

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 6

ER -

ID: 129809843