Synthetic lethality in the tobacco plastid ribosome and its rescue at elevated growth temperatures
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Synthetic lethality in the tobacco plastid ribosome and its rescue at elevated growth temperatures. / Ehrnthaler, Miriam; Scharff, Lars; Fleischmann, Tobias T; Hasse, Claudia; Ruf, Stephanie; Bock, Ralph.
I: Plant Cell, Bind 26, Nr. 2, 2014, s. 765-76.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Synthetic lethality in the tobacco plastid ribosome and its rescue at elevated growth temperatures
AU - Ehrnthaler, Miriam
AU - Scharff, Lars
AU - Fleischmann, Tobias T
AU - Hasse, Claudia
AU - Ruf, Stephanie
AU - Bock, Ralph
PY - 2014
Y1 - 2014
N2 - Consistent with their origin from cyanobacteria, plastids (chloroplasts) perform protein biosynthesis on bacterial-type 70S ribosomes. The plastid genomes of seed plants contain a conserved set of ribosomal protein genes. Three of these have proven to be nonessential for translation and, thus, for cellular viability: rps15, rpl33, and rpl36. To help define the minimum ribosome, here, we examined whether more than one of these nonessential plastid ribosomal proteins can be removed from the 70S ribosome. To that end, we constructed all possible double knockouts for the S15, L33, and L36 ribosomal proteins by stable transformation of the tobacco (Nicotiana tabacum) plastid genome. We find that, although S15 and L33 function in different ribosomal particles (30S and 50S, respectively), their combined deletion from the plastid genome results in synthetic lethality under autotrophic conditions. Interestingly, the lethality can be overcome by growth under elevated temperatures due to an improved efficiency of plastid ribosome biogenesis. Our results reveal functional interactions between protein and RNA components of the 70S ribosome and uncover the interdependence of the biogenesis of the two ribosomal subunits. In addition, our findings suggest that defining a minimal set of plastid genes may prove more complex than generally believed.
AB - Consistent with their origin from cyanobacteria, plastids (chloroplasts) perform protein biosynthesis on bacterial-type 70S ribosomes. The plastid genomes of seed plants contain a conserved set of ribosomal protein genes. Three of these have proven to be nonessential for translation and, thus, for cellular viability: rps15, rpl33, and rpl36. To help define the minimum ribosome, here, we examined whether more than one of these nonessential plastid ribosomal proteins can be removed from the 70S ribosome. To that end, we constructed all possible double knockouts for the S15, L33, and L36 ribosomal proteins by stable transformation of the tobacco (Nicotiana tabacum) plastid genome. We find that, although S15 and L33 function in different ribosomal particles (30S and 50S, respectively), their combined deletion from the plastid genome results in synthetic lethality under autotrophic conditions. Interestingly, the lethality can be overcome by growth under elevated temperatures due to an improved efficiency of plastid ribosome biogenesis. Our results reveal functional interactions between protein and RNA components of the 70S ribosome and uncover the interdependence of the biogenesis of the two ribosomal subunits. In addition, our findings suggest that defining a minimal set of plastid genes may prove more complex than generally believed.
KW - Gene Knockout Techniques
KW - Genes, Plant
KW - Mutation
KW - Phenotype
KW - Plant Proteins
KW - Plastids
KW - Polyribosomes
KW - Protein Biosynthesis
KW - RNA Processing, Post-Transcriptional
KW - Ribosomal Proteins
KW - Ribosomes
KW - Seedling
KW - Temperature
KW - Tobacco
U2 - 10.1105/tpc.114.123240
DO - 10.1105/tpc.114.123240
M3 - Journal article
C2 - 24563204
VL - 26
SP - 765
EP - 776
JO - The Plant Cell
JF - The Plant Cell
SN - 1040-4651
IS - 2
ER -
ID: 144532489