Synthesis of a polyhistidine-bearing amphipol and its use for immobilizing membrane proteins

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Fabrice Giusti
  • Pascal Kessler
  • Randi Westh Hansen
  • Eduardo Antonio Della Pia
  • Christel Le Bon
  • Gilles Mourier
  • Jean-Luc Popot
  • Martinez, Karen Laurence
  • Manuela Zoonens

Amphipols (APols) are short amphipathic polymers that stabilize membrane proteins (MPs) in aqueous solutions. In the present study, A8-35, a polyacrylate-based APol, was grafted with hexahistidine tags (His6-tags). The synthesis and characterization of this novel functionalized APol, named HistAPol, are described. Its ability to immobilize MPs on nickel ion-bearing surfaces was tested using two complementary methods, immobilized metal affinity chromatography (IMAC) and surface plasmon resonance (SPR). Compared to a single His6-tag fused at one extremity of a MP, the presence of several His6-tags carried by the APol belt surrounding the transmembrane domain of a MP increases remarkably the affinity of the protein/APol complex for nickel ion-bearing SPR chips, whereas it does not show such a strong effect on an IMAC resin. HistAPol-mediated immobilization, which allows reversibility of the interaction and easy regeneration of the supports and dispenses with any genetic modification of the target protein, provides a novel, promising tool for attaching MPs onto solid supports while stabilizing them.

Udgave nummer12
Sider (fra-til)3751-3761
Antal sider11
StatusUdgivet - 2015

ID: 147934539