Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Lipid-conjugated pH sensors based on fluorophores coupled to lipids are a powerful tool for monitoring pH gradients in biological microcompartments and reconstituted membrane systems. This protocol describes the synthesis of pH sensors based on amine-reactive pHrodo esters and the amino phospholipid phosphatidylethanolamine. The major features of this sensor include efficient partitioning into membranes and strong fluorescence under acidic conditions. The protocol described here can be used as a template to couple other amine-reactive fluorophores to phosphatidylethanolamines.
Originalsprog | Engelsk |
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Artikelnummer | e4694 |
Tidsskrift | Bio-protocol |
Vol/bind | 13 |
Udgave nummer | 11 |
ISSN | 2331-8325 |
DOI | |
Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:
This protocol was adapted from our previous work (Kemmer et al., 2015; Veshaguri et al., 2016; Kühnel et al., 2019; Bohr et al., 2020). We would like to thank PD Dr. Jürgen Schiller (University of Leipzig, Germany) for his continuous help with mass spectroscopy during the whole time of this project. This work was supported by an instrument grant from the Deutsche Forschungsgemeinschaft (INST 213/886-1 FUGG, INST 213/985-1FUGG; GU 1133/11-1). The graphical abstract was created using BioRender.com.
Publisher Copyright:
© 2023 The Authors.
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