Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis
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Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long-held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects, or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared with wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirements for cellulose biosynthesis in Arabidopsis can be refuted.
Originalsprog | Engelsk |
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Tidsskrift | Plant Journal |
Vol/bind | 110 |
Udgave nummer | 5 |
Sider (fra-til) | 1493-1497 |
ISSN | 0960-7412 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
We thank Junko Takahashi‐Schmidt and the biopolymer analytical facility at UPSC for help with cellulose analysis. This work was supported by Bio4Energy (Swedish Programme for Renewable Energy), the Umeå Plant Science Centre, Berzelii Centre for Forest Biotechnology funded by VINNOVA, and the Swedish Research Council for Sustainable Development (Formas).
Publisher Copyright:
© 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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