Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Standard

Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions. / Adams, Yvonne.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Bind 2470 Springer, 2022. s. 537-543 (Methods in molecular biology (Clifton, N.J.)).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Adams, Y 2022, Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions. i Malaria Immunology: Targeting the Surface of Infected Erythrocytes. bind 2470, Springer, Methods in molecular biology (Clifton, N.J.), s. 537-543. https://doi.org/10.1007/978-1-0716-2189-9_41

APA

Adams, Y. (2022). Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions. I Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Bind 2470, s. 537-543). Springer. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_41

Vancouver

Adams Y. Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions. I Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Bind 2470. Springer. 2022. s. 537-543. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_41

Author

Adams, Yvonne. / Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Bind 2470 Springer, 2022. s. 537-543 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{18b05fd81e914f57afcda71c1e900cad,
title = "Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions",
abstract = "The var genes encoding the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) mediating adhesion of infected erythrocytes (IEs) are clonally variant and confer the parasite the ability to evade the host immune response by enabling switching among expression of different PfEMP1 variants. This method of antigenic variation allows the parasite to adhere to a variety of proteins to escape splenic clearance. Enriching IE populations for expression of a specific PfEMP1 variant is crucial for the study of specific var genes PfEMP1 proteins and their role in pathogenicity. Selection of parasites using cell monolayers requires large-volume cultures (>5 mL). This chapter describes a method, which rapidly selects for adhesive phenotypes of interest using small-volume cultures (<1 mL) under physiological flow conditions.",
keywords = "Animals, Antigenic Variation, Antigens, Protozoan/genetics, Erythrocytes/metabolism, Malaria, Falciparum/parasitology, Parasites/metabolism, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism",
author = "Yvonne Adams",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_41",
language = "English",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "537--543",
booktitle = "Malaria Immunology",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions

AU - Adams, Yvonne

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - The var genes encoding the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) mediating adhesion of infected erythrocytes (IEs) are clonally variant and confer the parasite the ability to evade the host immune response by enabling switching among expression of different PfEMP1 variants. This method of antigenic variation allows the parasite to adhere to a variety of proteins to escape splenic clearance. Enriching IE populations for expression of a specific PfEMP1 variant is crucial for the study of specific var genes PfEMP1 proteins and their role in pathogenicity. Selection of parasites using cell monolayers requires large-volume cultures (>5 mL). This chapter describes a method, which rapidly selects for adhesive phenotypes of interest using small-volume cultures (<1 mL) under physiological flow conditions.

AB - The var genes encoding the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) mediating adhesion of infected erythrocytes (IEs) are clonally variant and confer the parasite the ability to evade the host immune response by enabling switching among expression of different PfEMP1 variants. This method of antigenic variation allows the parasite to adhere to a variety of proteins to escape splenic clearance. Enriching IE populations for expression of a specific PfEMP1 variant is crucial for the study of specific var genes PfEMP1 proteins and their role in pathogenicity. Selection of parasites using cell monolayers requires large-volume cultures (>5 mL). This chapter describes a method, which rapidly selects for adhesive phenotypes of interest using small-volume cultures (<1 mL) under physiological flow conditions.

KW - Animals

KW - Antigenic Variation

KW - Antigens, Protozoan/genetics

KW - Erythrocytes/metabolism

KW - Malaria, Falciparum/parasitology

KW - Parasites/metabolism

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_41

DO - 10.1007/978-1-0716-2189-9_41

M3 - Book chapter

C2 - 35881373

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 537

EP - 543

BT - Malaria Immunology

PB - Springer

ER -

ID: 317092283