Screening archaeological bone for palaeogenetic and palaeoproteomic studies

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Screening archaeological bone for palaeogenetic and palaeoproteomic studies. / Kontopoulos, Ioannis; Penkman, Kirsty; Mullin, Victoria E.; Winkelbach, Laura; Unterländer, Martina; Scheu, Amelie; Kreutzer, Susanne; Hansen, Henrik B.; Margaryan, Ashot; Teasdale, Matthew D.; Gehlen, Birgit; Street, Martin; Lynnerup, Niels; Liritzis, Ioannis; Sampson, Adamantios; Papageorgopoulou, Christina; Allentoft, Morten E.; Burger, Joachim; Bradley, Daniel G.; Collins, Matthew J.

I: PLoS ONE, Bind 15, Nr. 6, e0235146, 06.2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kontopoulos, I, Penkman, K, Mullin, VE, Winkelbach, L, Unterländer, M, Scheu, A, Kreutzer, S, Hansen, HB, Margaryan, A, Teasdale, MD, Gehlen, B, Street, M, Lynnerup, N, Liritzis, I, Sampson, A, Papageorgopoulou, C, Allentoft, ME, Burger, J, Bradley, DG & Collins, MJ 2020, 'Screening archaeological bone for palaeogenetic and palaeoproteomic studies', PLoS ONE, bind 15, nr. 6, e0235146. https://doi.org/10.1371/journal.pone.0235146

APA

Kontopoulos, I., Penkman, K., Mullin, V. E., Winkelbach, L., Unterländer, M., Scheu, A., Kreutzer, S., Hansen, H. B., Margaryan, A., Teasdale, M. D., Gehlen, B., Street, M., Lynnerup, N., Liritzis, I., Sampson, A., Papageorgopoulou, C., Allentoft, M. E., Burger, J., Bradley, D. G., & Collins, M. J. (2020). Screening archaeological bone for palaeogenetic and palaeoproteomic studies. PLoS ONE, 15(6), [e0235146]. https://doi.org/10.1371/journal.pone.0235146

Vancouver

Kontopoulos I, Penkman K, Mullin VE, Winkelbach L, Unterländer M, Scheu A o.a. Screening archaeological bone for palaeogenetic and palaeoproteomic studies. PLoS ONE. 2020 jun.;15(6). e0235146. https://doi.org/10.1371/journal.pone.0235146

Author

Kontopoulos, Ioannis ; Penkman, Kirsty ; Mullin, Victoria E. ; Winkelbach, Laura ; Unterländer, Martina ; Scheu, Amelie ; Kreutzer, Susanne ; Hansen, Henrik B. ; Margaryan, Ashot ; Teasdale, Matthew D. ; Gehlen, Birgit ; Street, Martin ; Lynnerup, Niels ; Liritzis, Ioannis ; Sampson, Adamantios ; Papageorgopoulou, Christina ; Allentoft, Morten E. ; Burger, Joachim ; Bradley, Daniel G. ; Collins, Matthew J. / Screening archaeological bone for palaeogenetic and palaeoproteomic studies. I: PLoS ONE. 2020 ; Bind 15, Nr. 6.

Bibtex

@article{e4da6de45dfa445eb02353f2dcd43f59,
title = "Screening archaeological bone for palaeogenetic and palaeoproteomic studies",
abstract = "The recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. We establish thresholds for three different FTIR indices, a) the infrared splitting factor [IRSF] that assesses relative changes in bioapatite crystals{\textquoteright} size and homogeneity; b) the carbonate-to-phosphate [C/P] ratio as a relative measure of carbonate content in bioapatite crystals; and c) the amide-to-phosphate ratio [Am/P] for assessing the relative organic content preserved in bone. These thresholds are both extremely reliable and easy to apply for the successful and rapid distinction between well- and poorly-preserved specimens. This is a milestone for choosing appropriate samples prior to genomic and collagen analyses, with important implications for biomolecular archaeology and palaeontology.",
author = "Ioannis Kontopoulos and Kirsty Penkman and Mullin, {Victoria E.} and Laura Winkelbach and Martina Unterl{\"a}nder and Amelie Scheu and Susanne Kreutzer and Hansen, {Henrik B.} and Ashot Margaryan and Teasdale, {Matthew D.} and Birgit Gehlen and Martin Street and Niels Lynnerup and Ioannis Liritzis and Adamantios Sampson and Christina Papageorgopoulou and Allentoft, {Morten E.} and Joachim Burger and Bradley, {Daniel G.} and Collins, {Matthew J.}",
year = "2020",
month = jun,
doi = "10.1371/journal.pone.0235146",
language = "English",
volume = "15",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - Screening archaeological bone for palaeogenetic and palaeoproteomic studies

AU - Kontopoulos, Ioannis

AU - Penkman, Kirsty

AU - Mullin, Victoria E.

AU - Winkelbach, Laura

AU - Unterländer, Martina

AU - Scheu, Amelie

AU - Kreutzer, Susanne

AU - Hansen, Henrik B.

AU - Margaryan, Ashot

AU - Teasdale, Matthew D.

AU - Gehlen, Birgit

AU - Street, Martin

AU - Lynnerup, Niels

AU - Liritzis, Ioannis

AU - Sampson, Adamantios

AU - Papageorgopoulou, Christina

AU - Allentoft, Morten E.

AU - Burger, Joachim

AU - Bradley, Daniel G.

AU - Collins, Matthew J.

PY - 2020/6

Y1 - 2020/6

N2 - The recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. We establish thresholds for three different FTIR indices, a) the infrared splitting factor [IRSF] that assesses relative changes in bioapatite crystals’ size and homogeneity; b) the carbonate-to-phosphate [C/P] ratio as a relative measure of carbonate content in bioapatite crystals; and c) the amide-to-phosphate ratio [Am/P] for assessing the relative organic content preserved in bone. These thresholds are both extremely reliable and easy to apply for the successful and rapid distinction between well- and poorly-preserved specimens. This is a milestone for choosing appropriate samples prior to genomic and collagen analyses, with important implications for biomolecular archaeology and palaeontology.

AB - The recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. We establish thresholds for three different FTIR indices, a) the infrared splitting factor [IRSF] that assesses relative changes in bioapatite crystals’ size and homogeneity; b) the carbonate-to-phosphate [C/P] ratio as a relative measure of carbonate content in bioapatite crystals; and c) the amide-to-phosphate ratio [Am/P] for assessing the relative organic content preserved in bone. These thresholds are both extremely reliable and easy to apply for the successful and rapid distinction between well- and poorly-preserved specimens. This is a milestone for choosing appropriate samples prior to genomic and collagen analyses, with important implications for biomolecular archaeology and palaeontology.

U2 - 10.1371/journal.pone.0235146

DO - 10.1371/journal.pone.0235146

M3 - Journal article

C2 - 32584871

AN - SCOPUS:85087099665

VL - 15

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

M1 - e0235146

ER -

ID: 244496286