Sars-cov-2 in saliva, oropharyngeal and nasopharyngeal specimens
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Sars-cov-2 in saliva, oropharyngeal and nasopharyngeal specimens. / Todsen, Tobias; Tolsgaard, Martin; Folke, Fredrik; Jakobsen, Kathrine Kronberg; Ersbøll, Annette Kjær; Benfield, Thomas; Buchwald, Christian von; Kirkby, Nikolai.
I: Danish Medical Journal, Bind 68, Nr. 5, A01210087, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Sars-cov-2 in saliva, oropharyngeal and nasopharyngeal specimens
AU - Todsen, Tobias
AU - Tolsgaard, Martin
AU - Folke, Fredrik
AU - Jakobsen, Kathrine Kronberg
AU - Ersbøll, Annette Kjær
AU - Benfield, Thomas
AU - Buchwald, Christian von
AU - Kirkby, Nikolai
N1 - Publisher Copyright: © 2021, Almindelige Danske Laegeforening. All rights reserved.
PY - 2021
Y1 - 2021
N2 - INTRODUCTION: The reference test to evaluate patients with suspected respiratory virus infection is a real-time reverse transcription-polymerase chain reaction (RT-PCR) from a nasopharyngeal swab (NPS). However, other specimen collection methods such as an oropharyngeal swab (OPS) or saliva specimen are also used for SARS-CoV-2 testing during the ongoing COVID-19 pandemic. However, it remains unclear if rates of SARS-CoV-2 detection differ between sampling methods. This study will compare the rates of SARS-CoV-2 detection by saliva, OPS, and NPS sampling in a public setting. METHODS: Individuals referred for outpatient SARS-CoV-2 testing will be invited to participate in a prospective clinical study. They will have saliva, OPS and NPS specimens collected that will be analysed separately for SARS-CoV-2 RNA by RT-PCR. The rate of SARS-CoV-2 detection in saliva, OPS and NPS will be compared using a logistic regression mixed-effect model analysis. A sample of 19,110 participants is required at an expected 1.5% test-positive rate in order to detect a 25.6% difference. The total sample size will be adjusted as the test-positive rate changes. CONCLUSIONS: This study will provide evidence for the optimal site of specimen collection to detect SARS-CoV-2. The results may help guide the health authorities.
AB - INTRODUCTION: The reference test to evaluate patients with suspected respiratory virus infection is a real-time reverse transcription-polymerase chain reaction (RT-PCR) from a nasopharyngeal swab (NPS). However, other specimen collection methods such as an oropharyngeal swab (OPS) or saliva specimen are also used for SARS-CoV-2 testing during the ongoing COVID-19 pandemic. However, it remains unclear if rates of SARS-CoV-2 detection differ between sampling methods. This study will compare the rates of SARS-CoV-2 detection by saliva, OPS, and NPS sampling in a public setting. METHODS: Individuals referred for outpatient SARS-CoV-2 testing will be invited to participate in a prospective clinical study. They will have saliva, OPS and NPS specimens collected that will be analysed separately for SARS-CoV-2 RNA by RT-PCR. The rate of SARS-CoV-2 detection in saliva, OPS and NPS will be compared using a logistic regression mixed-effect model analysis. A sample of 19,110 participants is required at an expected 1.5% test-positive rate in order to detect a 25.6% difference. The total sample size will be adjusted as the test-positive rate changes. CONCLUSIONS: This study will provide evidence for the optimal site of specimen collection to detect SARS-CoV-2. The results may help guide the health authorities.
M3 - Journal article
C2 - 33832565
AN - SCOPUS:85105039410
VL - 68
JO - Danish Medical Journal
JF - Danish Medical Journal
SN - 2245-1919
IS - 5
M1 - A01210087
ER -
ID: 262801966