Robustness and stability of the gene regulatory network involved in DV boundary formation in the Drosophila wing
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Robustness and stability of the gene regulatory network involved in DV boundary formation in the Drosophila wing. / Buceta, Javier; Herranz, Héctor; Canela-Xandri, Oriol; Reigada, Ramon; Sagués, Francesc; Milán, Marco.
I: PLOS ONE, Bind 2, Nr. 7, 2007, s. e602.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Robustness and stability of the gene regulatory network involved in DV boundary formation in the Drosophila wing
AU - Buceta, Javier
AU - Herranz, Héctor
AU - Canela-Xandri, Oriol
AU - Reigada, Ramon
AU - Sagués, Francesc
AU - Milán, Marco
PY - 2007
Y1 - 2007
N2 - Gene regulatory networks have been conserved during evolution. The Drosophila wing and the vertebrate hindbrain share the gene network involved in the establishment of the boundary between dorsal and ventral compartments in the wing and adjacent rhombomeres in the hindbrain. A positive feedback-loop between boundary and non-boundary cells and mediated by the activities of Notch and Wingless/Wnt-1 leads to the establishment of a Notch dependent organizer at the boundary. By means of a Systems Biology approach that combines mathematical modeling and both in silico and in vivo experiments in the Drosophila wing primordium, we modeled and tested this regulatory network and present evidence that a novel property, namely refractoriness to the Wingless signaling molecule, is required in boundary cells for the formation of a stable dorsal-ventral boundary. This new property has been validated in vivo, promotes mutually exclusive domains of Notch and Wingless activities and confers stability to the dorsal-ventral boundary. A robustness analysis of the regulatory network complements our results and ensures its biological plausibility.
AB - Gene regulatory networks have been conserved during evolution. The Drosophila wing and the vertebrate hindbrain share the gene network involved in the establishment of the boundary between dorsal and ventral compartments in the wing and adjacent rhombomeres in the hindbrain. A positive feedback-loop between boundary and non-boundary cells and mediated by the activities of Notch and Wingless/Wnt-1 leads to the establishment of a Notch dependent organizer at the boundary. By means of a Systems Biology approach that combines mathematical modeling and both in silico and in vivo experiments in the Drosophila wing primordium, we modeled and tested this regulatory network and present evidence that a novel property, namely refractoriness to the Wingless signaling molecule, is required in boundary cells for the formation of a stable dorsal-ventral boundary. This new property has been validated in vivo, promotes mutually exclusive domains of Notch and Wingless activities and confers stability to the dorsal-ventral boundary. A robustness analysis of the regulatory network complements our results and ensures its biological plausibility.
KW - Animals
KW - Drosophila
KW - Drosophila Proteins
KW - Feedback
KW - Gene Expression Regulation
KW - Homeostasis
KW - Ligands
KW - Protein Biosynthesis
KW - Receptors, Cell Surface
KW - Transcription, Genetic
KW - Vertebrates
KW - Wing
KW - Wnt1 Protein
U2 - 10.1371/journal.pone.0000602
DO - 10.1371/journal.pone.0000602
M3 - Journal article
C2 - 17622347
VL - 2
SP - e602
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 7
ER -
ID: 119179743