Reduced ex vivo stimulated IL-6 response in infants randomized to fish oil from 9 to 18 months, especially among PPARG2 and COX2 wild types
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Reduced ex vivo stimulated IL-6 response in infants randomized to fish oil from 9 to 18 months, especially among PPARG2 and COX2 wild types. / Harsløf, Laurine Bente Schram; Damsgaard, Camilla Trab; Andersen, Anders D; Aakjær, Ditte L; Michaelsen, Kim F.; Hellgren, Lars I; Frøkiær, Hanne; Vogel, Ulla; Lauritzen, Lotte.
I: Prostaglandins, Leukotrienes & Essential Fatty Acids, Bind 94, Nr. 1, 2015, s. 21-27.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Reduced ex vivo stimulated IL-6 response in infants randomized to fish oil from 9 to 18 months, especially among PPARG2 and COX2 wild types
AU - Harsløf, Laurine Bente Schram
AU - Damsgaard, Camilla Trab
AU - Andersen, Anders D
AU - Aakjær, Ditte L
AU - Michaelsen, Kim F.
AU - Hellgren, Lars I
AU - Frøkiær, Hanne
AU - Vogel, Ulla
AU - Lauritzen, Lotte
N1 - CURIS 2015 NEXS 039
PY - 2015
Y1 - 2015
N2 - We investigated whether n-3 LCPUFA affected immune function in late infancy and explored effect-modification by single nucleotide polymorphisms (SNPs) and links to intestinal microbiota. Infants (n=105) were randomized to fish oil (FO, 1.2g/d n-3 LCPUFA) or sunflower oil (SO)-supplements from age 9-18 months. Immune function was assessed by ex vivo cytokine production in stimulated blood and plasma immunoglobulin E (IgE). We genotyped functional SNPs in PPARG2 and COX2 and analyzed fecal microbiota by 16S-rRNA terminal restriction fragment length polymorphism. FO compared to SO reduced Lactobacillus paracasei-stimulated IL-6 at 18 months (P=0.03, n=104). This effect was most pronounced among infants wild-type for PPARG2-Pro12Ala and/or COX2-T8473C (P<0.05). Predominant bacterial fragments were associated with 18 months IgE in all infants (P=0.004) (bp100) and with IL-6 production among infants weaned before 9 months (P=0.047) (bp102). Thus, FO reduced IL-6 in a genotype-modified manner. The microbiota was partly linked to IL-6 and IgE, not directly to FO.
AB - We investigated whether n-3 LCPUFA affected immune function in late infancy and explored effect-modification by single nucleotide polymorphisms (SNPs) and links to intestinal microbiota. Infants (n=105) were randomized to fish oil (FO, 1.2g/d n-3 LCPUFA) or sunflower oil (SO)-supplements from age 9-18 months. Immune function was assessed by ex vivo cytokine production in stimulated blood and plasma immunoglobulin E (IgE). We genotyped functional SNPs in PPARG2 and COX2 and analyzed fecal microbiota by 16S-rRNA terminal restriction fragment length polymorphism. FO compared to SO reduced Lactobacillus paracasei-stimulated IL-6 at 18 months (P=0.03, n=104). This effect was most pronounced among infants wild-type for PPARG2-Pro12Ala and/or COX2-T8473C (P<0.05). Predominant bacterial fragments were associated with 18 months IgE in all infants (P=0.004) (bp100) and with IL-6 production among infants weaned before 9 months (P=0.047) (bp102). Thus, FO reduced IL-6 in a genotype-modified manner. The microbiota was partly linked to IL-6 and IgE, not directly to FO.
U2 - 10.1016/j.plefa.2014.10.007
DO - 10.1016/j.plefa.2014.10.007
M3 - Journal article
C2 - 25498245
VL - 94
SP - 21
EP - 27
JO - Prostaglandins, Leukotrienes & Essential Fatty Acids
JF - Prostaglandins, Leukotrienes & Essential Fatty Acids
SN - 0952-3278
IS - 1
ER -
ID: 130294827