Receptor affinity-based purification of PfEMP1 proteins
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
The virulence of Plasmodium falciparum is linked to the ability of infected erythrocytes (IEs) to bind a range of human receptors. This binding is mediated by a family of highly polymorphic proteins known as P. falciparum erythrocyte membrane protein 1 (PfEMP1). PfEMP1 proteins are expressed on the surface of IEs and are composed of extracellular domains (NTS, CIDR, DBL), a transmembrane region and an acidic C-terminal segment. Subdomains of the extracellular N-terminal part of PfEMP1 molecules have been shown to bind specific receptors.In this chapter, we describe how to purify PfEMP1 proteins by a receptor affinity-based method. This includes how to prepare affinity columns and how to subsequently test the functionality of the purified PfEMP1 protein in an ELISA-based assay.
Originalsprog | Engelsk |
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Titel | Malaria Immunology : Targeting the Surface of Infected Erythrocytes |
Antal sider | 10 |
Vol/bind | 2470 |
Forlag | Humana Press |
Publikationsdato | 2022 |
Sider | 299-308 |
ISBN (Elektronisk) | 978-1-0716-2189-9 |
DOI | |
Status | Udgivet - 2022 |
Navn | Methods in molecular biology (Clifton, N.J.) |
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ISSN | 1064-3745 |
Bibliografisk note
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ID: 320648912