TY - JOUR

T1 - Rapid and Wash-Free Time-Gated FRET Histamine Assays Using Antibodies and Aptamers

AU - Fu, Hui Jun

AU - Su, Ruifang

AU - Luo, Lin

AU - Chen, Zi Jian

AU - Sørensen, Thomas Just

AU - Hildebrandt, Niko

AU - Xu, Zhen Lin

N1 - Funding Information: We thank Lumiphore, Inc. for the gift of Lumi4 reagents. This work was supported by China Scholarship Council (CSC), National Nature Science Foundation of China (31822039), National Research Foundation of Korea, Seoul National University, University of Copenhagen, Villum Fonden (grant#14922), Université Paris-Saclay, Université de Rouen Normandie, INSA Rouen, CNRS, Normandie Université, European Regional Development Fund, Labex SynOrg (ANR-11-LABX-0029), Carnot Institute I2C, XL-Chem graduate school (ANR-18-EURE-0020 XL CHEM), and Région Normandie. Publisher Copyright: © 2022 American Chemical Society. All rights reserved.

PY - 2022

Y1 - 2022

N2 - Histamine (HA) is an indicator of food freshness and quality. However, high concentrations of HA can cause food poisoning. Simple, rapid, sensitive, and specific quantification can enable efficient screening of HA in food and beverages. However, conventional assays are complicated and time-consuming, as they require multiple incubation, washing, and separation steps. Here, we demonstrate that time-gated Förster resonance energy transfer (TG-FRET) between terbium (Tb) complexes and organic dyes can be implemented in both immunosensors and aptasensors for simple HA quantification using a rapid, single-step, mix-and-measure assay format. Both biosensors could quantify HA at concentrations relevant in food poisoning with limits of detection of 0.19 μg/mL and 0.03 μg/mL, respectively. Excellent specificity was documented against the structurally similar food components tryptamine and l-histidine. Direct applicability of the TG-FRET assays was demonstrated by quantifying HA in spiked fish and wine samples with both excellent concentration recovery and agreement with conventional multistep enzyme-linked immunosorbent assays (ELISAs). Our results show that the simplicity and rapidity of TG-FRET assays do not compromise sensitivity, specificity, and reliability, and both immunosensors and aptasensors have a strong potential for their implementation in advanced food safety screening.

AB - Histamine (HA) is an indicator of food freshness and quality. However, high concentrations of HA can cause food poisoning. Simple, rapid, sensitive, and specific quantification can enable efficient screening of HA in food and beverages. However, conventional assays are complicated and time-consuming, as they require multiple incubation, washing, and separation steps. Here, we demonstrate that time-gated Förster resonance energy transfer (TG-FRET) between terbium (Tb) complexes and organic dyes can be implemented in both immunosensors and aptasensors for simple HA quantification using a rapid, single-step, mix-and-measure assay format. Both biosensors could quantify HA at concentrations relevant in food poisoning with limits of detection of 0.19 μg/mL and 0.03 μg/mL, respectively. Excellent specificity was documented against the structurally similar food components tryptamine and l-histidine. Direct applicability of the TG-FRET assays was demonstrated by quantifying HA in spiked fish and wine samples with both excellent concentration recovery and agreement with conventional multistep enzyme-linked immunosorbent assays (ELISAs). Our results show that the simplicity and rapidity of TG-FRET assays do not compromise sensitivity, specificity, and reliability, and both immunosensors and aptasensors have a strong potential for their implementation in advanced food safety screening.

KW - aptasensors

KW - fluorescence

KW - food testing

KW - Förster resonance energy transfer

KW - immunoassays

U2 - 10.1021/acssensors.2c00085

DO - 10.1021/acssensors.2c00085

M3 - Journal article

C2 - 35312279

AN - SCOPUS:85127868209

VL - 7

SP - 1113

EP - 1121

JO - ACS Sensors

JF - ACS Sensors

SN - 2379-3694

IS - 4

ER -