Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.
Originalsprog | Engelsk |
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Titel | Methods in Molecular Biology |
Antal sider | 10 |
Forlag | Humana Press |
Publikationsdato | 2022 |
Sider | 75-84 |
DOI | |
Status | Udgivet - 2022 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2550 |
ISSN | 1064-3745 |
Bibliografisk note
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ID: 323006087