Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry

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Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).1

TidsskriftSTAR Protocols
Udgave nummer2
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This study was supported by the Lundbeck Foundation through grants R250-2017-633 (M.L.) and R105-A9791 (T.B.) and the Novo Nordisk Foundation through grants NNF19OC0056411 (T.B.), NNF19OC0054390 (T.B.), and NNF20OC0062047 (J.B.K.), as well as the Independent Research Fund Denmark through grant 9040-00023B (T.T.-N.).

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© 2023 The Authors

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