Production of anti-PfEMP1 polyclonal antisera in rats and mice

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Standard

Production of anti-PfEMP1 polyclonal antisera in rats and mice. / Olsen, Rebecca W; Suurbaar, Jennifer; Jensen, Anja Ramstedt.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. red. / Anja Tatiana Ramstedt Jensen; Lars Hviid. Bind 2470 Humana Press, 2022. s. 381-389 (Methods in molecular biology (Clifton, N.J.)).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Olsen, RW, Suurbaar, J & Jensen, AR 2022, Production of anti-PfEMP1 polyclonal antisera in rats and mice. i ATR Jensen & L Hviid (red), Malaria Immunology: Targeting the Surface of Infected Erythrocytes. bind 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), s. 381-389. https://doi.org/10.1007/978-1-0716-2189-9_28

APA

Olsen, R. W., Suurbaar, J., & Jensen, A. R. (2022). Production of anti-PfEMP1 polyclonal antisera in rats and mice. I A. T. R. Jensen, & L. Hviid (red.), Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Bind 2470, s. 381-389). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_28

Vancouver

Olsen RW, Suurbaar J, Jensen AR. Production of anti-PfEMP1 polyclonal antisera in rats and mice. I Jensen ATR, Hviid L, red., Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Bind 2470. Humana Press. 2022. s. 381-389. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_28

Author

Olsen, Rebecca W ; Suurbaar, Jennifer ; Jensen, Anja Ramstedt. / Production of anti-PfEMP1 polyclonal antisera in rats and mice. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. red. / Anja Tatiana Ramstedt Jensen ; Lars Hviid. Bind 2470 Humana Press, 2022. s. 381-389 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{5b232f75812f45178c6a7312a31c28f5,

title = "Production of anti-PfEMP1 polyclonal antisera in rats and mice",

abstract = "Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.",

keywords = "Animals, Antibodies, Protozoan, Endothelial Cells/metabolism, Erythrocytes/metabolism, Humans, Immune Sera/metabolism, Malaria, Falciparum, Mice, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism, Rats",

author = "Olsen, {Rebecca W} and Jennifer Suurbaar and Jensen, {Anja Ramstedt}",

note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2189-9_28",

language = "English",

volume = "2470",

series = "Methods in molecular biology (Clifton, N.J.)",

publisher = "Humana Press",

pages = "381--389",

editor = "Jensen, {Anja Tatiana Ramstedt } and Hviid, {Lars }",

booktitle = "Malaria Immunology",

address = "United States",

}

RIS

TY - CHAP

T1 - Production of anti-PfEMP1 polyclonal antisera in rats and mice

AU - Olsen, Rebecca W

AU - Suurbaar, Jennifer

AU - Jensen, Anja Ramstedt

PY - 2022

Y1 - 2022

N2 - Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.

AB - Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.

KW - Animals

KW - Antibodies, Protozoan

KW - Endothelial Cells/metabolism

KW - Erythrocytes/metabolism

KW - Humans

KW - Immune Sera/metabolism

KW - Malaria, Falciparum

KW - Mice

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

KW - Rats

U2 - 10.1007/978-1-0716-2189-9_28

DO - 10.1007/978-1-0716-2189-9_28

M3 - Book chapter

C2 - 35881360

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 381

EP - 389

BT - Malaria Immunology

A2 - Jensen, Anja Tatiana Ramstedt

A2 - Hviid, Lars

PB - Humana Press

ER -

ID: 327466186