Production of anti-PfEMP1 polyclonal antisera in rats and mice

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.

OriginalsprogEngelsk
TitelMalaria Immunology : Targeting the Surface of Infected Erythrocytes
RedaktørerAnja Tatiana Ramstedt Jensen, Lars Hviid
Antal sider9
Vol/bind2470
ForlagHumana Press
Publikationsdato2022
Sider381-389
ISBN (Elektronisk)978-1-0716-2189-9
DOI
StatusUdgivet - 2022
NavnMethods in molecular biology (Clifton, N.J.)
ISSN1064-3745

Bibliografisk note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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