Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice

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Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice. / Harms, Matthew J; Ishibashi, Jeff; Wang, Wenshan; Lim, Hee-Woong; Goyama, Susumu; Sato, Tomohiko; Kurokawa, Mineo; Won, Kyoung-Jae; Seale, Patrick.

I: Cell Metabolism, Bind 19, Nr. 4, 01.04.2014, s. 593-604.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Harms, MJ, Ishibashi, J, Wang, W, Lim, H-W, Goyama, S, Sato, T, Kurokawa, M, Won, K-J & Seale, P 2014, 'Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice', Cell Metabolism, bind 19, nr. 4, s. 593-604. https://doi.org/10.1016/j.cmet.2014.03.007

APA

Harms, M. J., Ishibashi, J., Wang, W., Lim, H-W., Goyama, S., Sato, T., Kurokawa, M., Won, K-J., & Seale, P. (2014). Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice. Cell Metabolism, 19(4), 593-604. https://doi.org/10.1016/j.cmet.2014.03.007

Vancouver

Harms MJ, Ishibashi J, Wang W, Lim H-W, Goyama S, Sato T o.a. Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice. Cell Metabolism. 2014 apr. 1;19(4):593-604. https://doi.org/10.1016/j.cmet.2014.03.007

Author

Harms, Matthew J ; Ishibashi, Jeff ; Wang, Wenshan ; Lim, Hee-Woong ; Goyama, Susumu ; Sato, Tomohiko ; Kurokawa, Mineo ; Won, Kyoung-Jae ; Seale, Patrick. / Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice. I: Cell Metabolism. 2014 ; Bind 19, Nr. 4. s. 593-604.

Bibtex

@article{7ef31ebec7104b12ad1fadd8fa5c202f,

title = "Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice",

abstract = "Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development. However, Prdm16 was required in young mice to suppress the expression of white-fat-selective genes in BAT through recruitment of the histone methyltransferase Ehmt1. Additionally, Prdm16 deficiency caused a severe adult-onset decline in the thermogenic character of interscapular BAT. This resulted in BAT dysfunction and cold sensitivity but did not predispose the animals to obesity. Interestingly, the loss of brown fat identity due to ablation of Prdm16 was accelerated by concurrent deletion of the closely related Prdm3 gene. Together, these results show that Prdm16 and Prdm3 control postnatal BAT identity and function.",

keywords = "Adipocytes, Brown/metabolism, Aging/physiology, Analysis of Variance, Animals, Blotting, Western, Chromatin Immunoprecipitation, DNA-Binding Proteins/genetics, Flow Cytometry, Gene Expression Regulation, Developmental/genetics, Histological Techniques, Histone-Lysine N-Methyltransferase/metabolism, Mice, Mice, Knockout, Microarray Analysis, Real-Time Polymerase Chain Reaction, Transcription Factors/genetics",

author = "Harms, {Matthew J} and Jeff Ishibashi and Wenshan Wang and Hee-Woong Lim and Susumu Goyama and Tomohiko Sato and Mineo Kurokawa and Kyoung-Jae Won and Patrick Seale",

year = "2014",

month = apr,

day = "1",

doi = "10.1016/j.cmet.2014.03.007",

language = "English",

volume = "19",

pages = "593--604",

journal = "Cell Metabolism",

issn = "1550-4131",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice

AU - Harms, Matthew J

AU - Ishibashi, Jeff

AU - Wang, Wenshan

AU - Lim, Hee-Woong

AU - Goyama, Susumu

AU - Sato, Tomohiko

AU - Kurokawa, Mineo

AU - Won, Kyoung-Jae

AU - Seale, Patrick

PY - 2014/4/1

Y1 - 2014/4/1

N2 - Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development. However, Prdm16 was required in young mice to suppress the expression of white-fat-selective genes in BAT through recruitment of the histone methyltransferase Ehmt1. Additionally, Prdm16 deficiency caused a severe adult-onset decline in the thermogenic character of interscapular BAT. This resulted in BAT dysfunction and cold sensitivity but did not predispose the animals to obesity. Interestingly, the loss of brown fat identity due to ablation of Prdm16 was accelerated by concurrent deletion of the closely related Prdm3 gene. Together, these results show that Prdm16 and Prdm3 control postnatal BAT identity and function.

AB - Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development. However, Prdm16 was required in young mice to suppress the expression of white-fat-selective genes in BAT through recruitment of the histone methyltransferase Ehmt1. Additionally, Prdm16 deficiency caused a severe adult-onset decline in the thermogenic character of interscapular BAT. This resulted in BAT dysfunction and cold sensitivity but did not predispose the animals to obesity. Interestingly, the loss of brown fat identity due to ablation of Prdm16 was accelerated by concurrent deletion of the closely related Prdm3 gene. Together, these results show that Prdm16 and Prdm3 control postnatal BAT identity and function.

KW - Adipocytes, Brown/metabolism

KW - Aging/physiology

KW - Analysis of Variance

KW - Animals

KW - Blotting, Western

KW - Chromatin Immunoprecipitation

KW - DNA-Binding Proteins/genetics

KW - Flow Cytometry

KW - Gene Expression Regulation, Developmental/genetics

KW - Histological Techniques

KW - Histone-Lysine N-Methyltransferase/metabolism

KW - Mice

KW - Mice, Knockout

KW - Microarray Analysis

KW - Real-Time Polymerase Chain Reaction

KW - Transcription Factors/genetics

U2 - 10.1016/j.cmet.2014.03.007

DO - 10.1016/j.cmet.2014.03.007

M3 - Journal article

C2 - 24703692

VL - 19

SP - 593

EP - 604

JO - Cell Metabolism

JF - Cell Metabolism

SN - 1550-4131

IS - 4

ER -

ID: 199332305