Plasticity of thoracic interneurones rostral to a lateral spinal cord lesion
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Plasticity of thoracic interneurones rostral to a lateral spinal cord lesion. / Meehan, Claire Francesca; Ford, Timothy W.; Kirkwood, Peter A.
I: Experimental Neurology, Bind 331, 113361, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Plasticity of thoracic interneurones rostral to a lateral spinal cord lesion
AU - Meehan, Claire Francesca
AU - Ford, Timothy W.
AU - Kirkwood, Peter A.
PY - 2020
Y1 - 2020
N2 - The morphology and projections of ventral horn interneurones in the segment above an ipsilateral thoracic lateral spinal cord lesion were studied in the cat by intracellular injections of Neurobiotin at 6 to 18 weeks post-lesion and compared with previously published control data from uninjured spinal cords. The cell axons ascended, descended or both, mostly contralaterally and mostly spared by the lesion. Unusual morphological dendritic features were seen in the lesion group, mostly growth-related, including complex dendritic appendages, twisted or multiple-branched terminal dendrites, commissural dendrites, apparently swollen proximal dendrites and rostrocaudal asymmetries. Significant quantitative differences included more dendritic spines in the lesion group (3.4 x) and smaller soma areas in the lesion group (with similar numbers of primary dendrites and rostrocaudal dendritic spans). Immunoreactivity to microtubule associated protein 2a/b was detected in the proximal, but not distal, dendrites of cells in the lesion group, corresponding to an overall decrease in immunoreactivity in the ventral horns on the lesion side compared to the other. For axon collaterals, significant increases for the lesion group were seen in the number of collaterals in the first 4 mm of axon and in the area of ventral/intermediate horn occupied by terminals, including increased innervation of some regions, among which were the intermediolateral columns. This dendritic and axonal plasticity makes the interneuones candidates for a role in detour circuits but also for a maladaptive role in autonomic hyperreflexia.
AB - The morphology and projections of ventral horn interneurones in the segment above an ipsilateral thoracic lateral spinal cord lesion were studied in the cat by intracellular injections of Neurobiotin at 6 to 18 weeks post-lesion and compared with previously published control data from uninjured spinal cords. The cell axons ascended, descended or both, mostly contralaterally and mostly spared by the lesion. Unusual morphological dendritic features were seen in the lesion group, mostly growth-related, including complex dendritic appendages, twisted or multiple-branched terminal dendrites, commissural dendrites, apparently swollen proximal dendrites and rostrocaudal asymmetries. Significant quantitative differences included more dendritic spines in the lesion group (3.4 x) and smaller soma areas in the lesion group (with similar numbers of primary dendrites and rostrocaudal dendritic spans). Immunoreactivity to microtubule associated protein 2a/b was detected in the proximal, but not distal, dendrites of cells in the lesion group, corresponding to an overall decrease in immunoreactivity in the ventral horns on the lesion side compared to the other. For axon collaterals, significant increases for the lesion group were seen in the number of collaterals in the first 4 mm of axon and in the area of ventral/intermediate horn occupied by terminals, including increased innervation of some regions, among which were the intermediolateral columns. This dendritic and axonal plasticity makes the interneuones candidates for a role in detour circuits but also for a maladaptive role in autonomic hyperreflexia.
KW - DORSAL-HORN INTERNEURONS
KW - RESPIRATORY INTERNEURONS
KW - AUTONOMIC DYSREFLEXIA
KW - PROPRIOSPINAL NEURONS
KW - FUNCTIONAL PLASTICITY
KW - AXONAL REGENERATION
KW - LOCOMOTOR RECOVERY
KW - INJURY
KW - RAT
KW - MOTONEURONS
U2 - 10.1016/j.expneurol.2020.113361
DO - 10.1016/j.expneurol.2020.113361
M3 - Journal article
C2 - 32464119
VL - 331
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
M1 - 113361
ER -
ID: 248801997